A transgenic approach to studying imprinted genes: modified BACs and PACs.
A transgenic approach to studying imprinted genes: modified BACs and PACs.
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研究印记基因的转基因方法：改良的 BAC 和 PAC。
DOI：
10.1385/1-59259-211-2:67
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发表时间：
2001
影响因子：
--
通讯作者：
S. Barton
中科院分区：
文献类型：
--
作者：
R. John;J. Ainscough;S. Barton
在第4章中描述了在印迹基因分析中使用大基因组克隆的优势，特别是酵母人工染色体（YAC）。它们平均含有500-600 kb的DNA，但可以更大（>1 Mb）。YACS在酵母中繁殖，因此可以通过同源重组进行遗传修饰，现在有许多使用它们来产生转基因小鼠的例子。本章描述了一个相对较新的策略，使用大型基因组克隆，依赖于大肠杆菌为基础的系统。
The advantages of using large genomic clones in the analysis of imprinted genes is described in Chapter 4 with particular reference to yeast artificial chromosomes (YACs). These contain on average 500-600 kb of DNA but can be much larger (>1 Mb). YACS are propagated in yeast and are therefore amenable to genetic modification by homologous recombination, and there are now many examples of their use to generate transgenic mice. This chapter describes a relatively new strategy for using large genomic clones that relies on Escherichia coli-based systems.