LINC00894 Enhances the Progression of Breast Cancer by Sponging miR-429 to Regulate ZEB1 Expression.
LINC00894 Enhances the Progression of Breast Cancer by Sponging miR-429 to Regulate ZEB1 Expression.
复制标题
DOI：
10.2147/ott.s277284
复制
发表时间：
2021
影响因子：
4
通讯作者：
Feng CB
中科院分区：
文献类型：
--
作者：
Meng DF;Shao H;Feng CB
已知长非编码RNA(LncRNAs)调节肿瘤的发生。虽然乳腺癌组织中有LINC00894的高表达，但其在乳腺癌进展中的具体生物学作用尚不清楚。本研究以LINC00894为研究对象，利用LINC00894基因芯片对乳腺癌组织中LncRNA的表达进行分析。采用定量逆转录-聚合酶链式反应检测45对乳腺癌组织和正常乳腺组织中LINC00894的表达，同时采用细胞计数试剂盒(CCK-8)、EDU法、集落形成实验和Transwell实验检测乳腺癌细胞的增殖和侵袭能力。采用双荧光素酶报告基因分析和生物信息学分析检测LINC00894的潜在靶点。此外，利用RNA结合蛋白免疫沉淀(RIP)和Western印迹分析来阐明其在乳腺癌进展调控中的相互作用和作用。LINC00894在乳腺癌细胞中高表达，其过表达显著促进了细胞的增殖和侵袭。此外，LINC00894通过与miR-429竞争性结合，正向调控ZEB1的表达。综上所述，这些结果表明，LINC00894竞争性地与miR-429结合，介导ZEB1的表达；因此，它可能在乳腺癌的发展中发挥作用。
Long non-coding RNAs (lncRNAs) are known to regulate tumorigenesis. Although breast cancer tissues show a high expression of LINC00894, its specific biological role in breast cancer progression is still unknown. In this study, lncRNA microarray was used to analyze the lncRNA expression in breast cancer tissues, and LINC00894 was selected for further analysis. Expression of LINC00894 in 45 pairs of breast cancer tissues and normal tissues obtained from patients with breast cancer was assessed by quantitative reverse transcription-PCR, while proliferation and invasion of breast cancer cells were assessed using a Cell Counting Kit-8 (CCK-8), EdU assay, colony formation experiment, and transwell assays. A dual-luciferase reporter gene assay and bioinformatics analysis were employed to detect potential targets of LINC00894. Additionally, RNA Binding Protein Immunoprecipitation (RIP) and Western blot assays were utilized to clarify its interaction and roles in the regulation of breast cancer progression. High expression of LINC00894 was observed in breast cancer cells, and its overexpression significantly expedited cell proliferation and invasion. Moreover, LINC00894 positively regulated the expression of ZEB1 by competitively binding to miR-429. Taken together, these results suggest that LINC00894 competitively binds to miR-429 to mediate ZEB1 expression; consequently, it is implicated to play a role in the progression of breast cancer.