TRANSSPLICING MUTANTS OF CHLAMYDOMONAS-REINHARDTII
TRANSSPLICING MUTANTS OF CHLAMYDOMONAS-REINHARDTII
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DOI：
10.1007/bf00264448
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发表时间：
1990-09-01
期刊：
影响因子：
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通讯作者：
ROCHAIX, JD
中科院分区：
文献类型：
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作者：
GOLDSCHMIDTCLERMONT, M;GIRARDBASCOU, J;ROCHAIX, JD
在莱茵衣藻中，psaA基因的三个外显子广泛地分散在叶绿体基因组上：外显子1和2处于相反的方向并且彼此远离并且远离外显子3。因此，成熟的mRNA，编码的核心多肽的光系统I，可能是组装从单独的前体通过剪接在反式。我们已经分离和表征了一组突变体，是缺乏在成熟的psaA mRNA。突变体属于14个核互补组和一个叶绿体位点所需的组装psaA mRNA。叶绿体基因座，tscA，是远离任何的外显子，必须编码一个因子所需的反式。突变体都显示只有三个表型，对应于一个或其他或两个加入反应中的缺陷之一。这些表型，和那些双突变体，是一致的存在两个选择性剪接途径。
In Chlamydomonas reinhardtii the three exons of the psaA gene are widely scattered on the chloroplast genome: exons 1 and 2 are in opposite orientations and distant from each other and from exon 3. The mature mRNA, encoding a core polypeptide of photosystem I, is thus probably assembled from separate precursors by splicing in trans. We have isolated and characterized a set of mutants that are deficient in the maturation of psaA mRNA. The mutants belong to 14 nuclear complementation groups and one chloroplast locus that are required for the assembly of psaA mRNA. The chloroplast locus, tscA, is remote from any of the exons and must encode a factor required in trans. The mutants all show one of only three phenotypes that correspond to defects in one or other or both of the joining reactions. These phenotypes, and those of double mutants, are consistent with the existence of two alternative splicing pathways.