Photosynthesis: X-ray investigation
Photosynthesis: X-ray investigation
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光合作用：X 射线研究
DOI：
10.1038/nphoton.2013.118
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发表时间：
2013
期刊：
影响因子：
35
通讯作者：
D. Pile
中科院分区：
文献类型：
--
作者：
D. Pile
X 射线衍射是 20 世纪 50 年代用于揭示 DNA 结构的材料分析技术，如今重新受到人们的关注，尤其是在蛋白质晶体学方面。原因之一是自由电子激光器的出现，它可以提供短而强的 X 射线脉冲。尽管 X 射线衍射可用于确定结构信息，但其他技术（例如探测电子能级的非共振 X 射线发射光谱）可以提供有关样品的附加信息。现在，Jan Kern 和来自美国、德国、瑞典和法国的同事使用美国斯坦福大学直线加速器相干光源产生的亚 50 飞秒 X 射线脉冲，在室温下对光系统 II 的微晶体进行同步 X 射线衍射和 X 射线发射光谱测量。光系统 II 是一种金属酶，在光合作用中发挥着关键作用；它存在于绿色植物、藻类和蓝细菌中。研究人员报告说，光系统 II 晶体在测量过程中保持完整，并希望该技术可用于光驱动结构变化的时间分辨研究。 DP
X-ray diffraction, the materials analysis technique used to reveal the structure of DNA in the 1950s, is receiving renewed interest, especially for protein crystallography. One reason for this is the advent of free-electron lasers, which can provide short, intense X-ray pulses. Although X-ray diffraction is useful for determining structural information, other techniques such as nonresonant X-ray emission spectroscopy, which probes electron levels, can provide additional information about a sample. Now, Jan Kern and colleagues from the USA, Germany, Sweden and France have used sub-50-fs X-ray pulses produced at the Linac Coherent Light Source at Stanford, USA, to conduct simultaneous X-ray diffraction and X-ray emission spectroscopy measurements of microcrystals of photosystem II at room temperature. Photosystem II is a metalloenzyme that plays a critical role in photosynthesis; it is found in green plants, algae and cyanobacteria. The researchers report that the photosystem II crystals remained intact during measurements and hope that the technique can be used for time-resolved studies of light-driven structural changes. DP