Ultrasensitive label-free electrochemical immunosensors for detecting marine medaka (Oryzias melastigma) vitellogenin based on novel Cu2O-BSA nanoparticles and anti-lipovitellin monoclonal antibody
Ultrasensitive label-free electrochemical immunosensors for detecting marine medaka (Oryzias melastigma) vitellogenin based on novel Cu2O-BSA nanoparticles and anti-lipovitellin monoclonal antibody
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基于新型 Cu2O-BSA 纳米粒子和抗卵黄蛋白单克隆抗体的超灵敏无标记电化学免疫传感器，用于检测海洋青鳉 (Oryzias melastigma) 卵黄原蛋白
DOI：
10.1016/j.snb.2021.130358
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yang Ailing
中科院分区：
文献类型：
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作者：
Zhang Zhenzhong;Ru Shaoguo;Wang Jun;Wang Dong;Zhao Lingchao;Teng Hayan;Dai Zhikuang;Zhang Wenzhe;Yang Ailing
卵黄蛋白原（Vtg）是环境雌激素的生物标志物，其灵敏的检测方法对于检测弱雌激素活性至关重要。本研究旨在开发基于新型纳米材料的海洋青鳉Vtg电化学免疫传感器。首先，合成直径为120 - 150 nm的Cu 2 O-BSA纳米颗粒（NPs），并制备抗卵黄脂磷蛋白的单克隆抗体（anti-Lv mAb）。以Cu 2 O-BSA纳米粒子和纳米金（Au NPs）为信号放大元件，抗Lv单克隆抗体为检测探针，建立了两种简单、高效的免标记免疫传感器。这两种免疫传感器具有良好的灵敏度、选择性、重复性和使用方便等优点。BSA/Anti-Lv mAb/Au/Cu 2 O-BSA/GCE免疫传感器的线性范围为0.128 ~ 5 × 104 pg/mL，最低检测限为0.09 pg/mL，大大低于以往报道的Vtg检测技术。最后，免疫传感器的性能进行了评估，通过定量Vtg浓度在血浆中的男性海洋青鳉暴露三种雌激素双酚。结果表明，BSA/Anti-Lv mAb/Au/Cu 2 O-BSA/GCE免疫传感器是一种操作简便、灵敏度高、准确度高的检测弱雌激素活性的方法。
Vitellogenin (Vtg) is a biomarker for environmental estrogens, and its sensitive detection method is vital for the detection of weak estrogenic activity. This study aimed to develop sensitive electrochemical immunosensors for marine medaka (Oryzias melastigma) Vtg based on novel nanomaterials. Firstly, Cu2O–BSA nanoparticles (NPs) with a diameter of 120−150 nm was synthesized, and monoclonal antibody against lipovitellin (anti-Lv mAb) was prepared. Then two simple and efficient label-free immunosensors for the detection of Vtg were established using Cu2O–BSA NPs and gold nanoparticle (Au NPs) as signal amplification elements and anti-Lv mAb as the detection probe. These two immunosensors have good sensitivity, selectivity, reproducibility for Vtg quantification, and are easy to use. Moreover, the BSA/Anti-Lv mAb/Au/Cu2O–BSA/GCE immunosensor exhibited a linear range from 0.128 to 5 × 104pg/mL, with the lowest detection limit (LOD) of 0.09 pg/mL, which is considerably lower than those of the previously reported Vtg detection techniques. Finally, the performance of the immunosensor was evaluated by quantifying Vtg concentrations in plasma of male marine medaka exposed three estrogenic bisphenols. The results demonstrated that the BSA/Anti-Lv mAb/Au/Cu2O–BSA/GCE immunosensor was an easily-operated, ultrasensitive and accurate detection method for weak estrogenic activity.