NEURONAL PRODUCTION, MIGRATION, AND DIFFERENTIATION IN A VOCAL CONTROL NUCLEUS OF THE ADULT FEMALE CANARY BRAIN
NEURONAL PRODUCTION, MIGRATION, AND DIFFERENTIATION IN A VOCAL CONTROL NUCLEUS OF THE ADULT FEMALE CANARY BRAIN
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DOI：
10.1073/pnas.80.8.2390
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
NOTTEBOHM, F
中科院分区：
文献类型：
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作者：
GOLDMAN, SA;NOTTEBOHM, F
成年雌性金丝雀的命名HVc（腹侧纹状体，尾部）的发声控制核扩大响应于系统性睾酮管理，这也诱导女性唱的男性样的方式。完整的成年雌性金丝雀注射[3H]胸苷超过2天的时间。一些鸟在胸苷之前的不同时间给予睾丸激素植入。在激素植入后5周处死这些鸟，并处理它们的脑用于放射自显影。在平行对照实验中，一些鸟被植入胆固醇而不是睾丸激素。所有的鸟都显示出相当数量的标记神经元，胶质细胞，内皮细胞，心室区细胞和周围HVc。超微结构分析证实了这些标记的神经元的身份。胆固醇和睾酮处理的鸟类有类似的神经元标记指数，其范围从1.8 - 4.0%的HVc。这些成年人的神经发生独立于外源性激素治疗。相反，胶质细胞和内皮细胞增殖率显着刺激外源性睾酮治疗。胸苷掺入神经元的起源通过在胸苷注射后不久处死2只胸苷处理的雌性动物来确定，排除新标记细胞的任何显著迁移。这些大脑的分析表明，没有细胞的神经元形态存在于HVc，但非常重标记的心室区覆盖HVc。神经元前体存在于HVc心室区，其在S期期间在其有丝分裂之前掺入氚化胸苷;分裂后，这些细胞迁移到HVc中并在一定程度上超出HVc。这种心室区神经发生似乎是一个正常发生的现象，在完整的成年雌性金丝雀。
The vocal control nucleus designated HVc (hyperstriatum ventrale, pars caudalis) of adult female canaries expands in response to systemic testosterone administration, which also induces the females to sing in a male-like manner. Intact adult female canaries were injected with [3H]thymidine over a 2-day period. Some birds were given testosterone implants at various times before thymidine. The birds were sacrificed 5 wk after hormone implantation, and their brains were processed for autoradiography. In parallel control experiments, some birds were given implants of cholesterol instead of testosterone. All birds showed considerable numbers of labeled neurons, glia, endothelia, and ventricular zone cells in and around HVc. Ultrastructural analysis confirmed the identity of these labeled neurons. Cholesterol and testosterone-treated birds had similar neuronal labeling indices, which ranged from 1.8-4.0% in HVc. Neurogenesis occurred in these adults independently of exogenous hormone treatment. Conversely, both glial and endothelial proliferation rates were markedly stimulated by exogenous testosterone treatment. The origin of the thymidine-incorporating neurons was determined by sacrificing 2 thymidine-treated females soon after their thymidine injections, precluding any significant migration of newly labeled cells. Analysis of these brains revealed no cells of neuronal morphology present in HVc but a very heavily labeled ventricular zone overlying HVc. Neuronal precursors exist in the HVc ventricular zone that incorporate tritiated thymidine during the S phase preceding their mitosis; after division these cells migrate into and to some extent beyond HVc. This ventricular zone neurogenesis seems to be a normally occurring phenomenon in intact adult female canaries.