Corneal stem cells in the eye clinic
Corneal stem cells in the eye clinic
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DOI：
10.1093/bmb/ldr041
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发表时间：
2011-12-01
影响因子：
6.7
通讯作者：
Daniels, Julie T.
中科院分区：
文献类型：
--
作者：
Shortt, Alex J.;Tuft, Stephen J.;Daniels, Julie T.
简介或背景：角膜混浊是失明的常见原因。大多数病例是由于感染或创伤后的溃疡和瘢痕形成，但在一定比例的角膜上皮干细胞（SC）缺乏导致无法维持健康的角膜表面。资料来源：本综述包括角膜上皮SC缺乏症的系统综述和个案系列治疗。一致的领域：比较了两种技术，如从健康眼睛移植大块角膜和在实验室中体外扩增角膜SCs。两者各有优点，临床结果相似。在细胞扩增方法中，较小的活检对供体眼睛的风险较小，这是一个显著的优势。争议领域：针对不同病因的SC失败的治疗算法正在不断发展。离体培养中真角膜上皮SCs的比例尚不清楚，也不清楚这些细胞是否能长期存活。生长点：在本研究中，细胞培养和移植的最佳方法正在深入研究中。及时开展研究的领域：使用多种细胞类型的组织的发展，遗传修饰治疗遗传性角膜疾病和其他眼病的细胞治疗的发展是未来的可能性。
Introduction or background: Corneal opacity is a common cause of blindness. The majority of cases result from ulceration and scarring following infection or trauma, but in a proportion corneal epithelial stem cell (SC) deficiency leads to an inability to maintain a healthy corneal surface.Sources of data: This review includes systematic reviews and individual case series of treatments for corneal epithelial SC deficiency.Areas of agreement: Two techniques such as transplantation of large segments of cornea from a healthy eye and ex vivo expansion of corneal SCs in the laboratory were compared. Both have merits and their clinical outcomes are similar. The smaller biopsy in the cell expansion approach has less risk for the donor eye, which is a significant advantage.Areas of controversy: Treatment algorithms for different aetiologies of SC failure are evolving. The proportion of true corneal epithelial SCs in ex vivo culture is unclear and it is unknown whether these cells survive long term.Growing points: In this study, the optimum method of cell culture and transplantation is being intensively investigated.Areas timely for developing research: Development of tissues using multiple cell types, genetic modification to treat hereditary corneal disorders and development of cell therapy for other eye diseases are future possibilities.