Alterations in Fetal Leydig Cell Gene Expression during Fetal and Adult Development
Alterations in Fetal Leydig Cell Gene Expression during Fetal and Adult Development
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DOI：
10.1159/000453323
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发表时间：
2017-01-01
影响因子：
2.3
通讯作者：
Morohashi, Ken-ichirou
中科院分区：
文献类型：
--
作者：
Miyabayashi, Kanako;Shima, Yuichi;Morohashi, Ken-ichirou
胎儿间质细胞（FLC）和成体间质细胞（ALCs）分别发育于哺乳动物出生前和出生后的睾丸。在小鼠中，FLC早在胚胎第12.5天就出现在睾丸的间隙中，此后在胎儿阶段数量增加。我们以前建立了一个转基因小鼠系，其中FLC标记的EGFP，并证明了EGFP标记的FLC存在，甚至在成年睾丸。然而，在出生后阶段的FLC的特点仍不清楚。在本研究中，产前和产后睾丸的FLCs和成人睾丸的ALCs的转录组的比较显示，FLCs逐渐改变其特征，在整个发育阶段，并大致类似ALCs。许多胆固醇基因同时表达一种独特的交替模式，而许多氧化磷酸化和β-氧化（线粒体功能）基因表现出不同的独特模式。这些代谢基因表达的改变可能是由环境的变化，如营养和氧气供应，从产前到产后的时期。(C)2016 S.Karger AG，巴塞尔
Fetal Leydig cells (FLCs) and adult Leydig cells (ALCs) develop in the mammalian prenatal and postnatal testes, respectively. In mice, FLCs emerge in the interstitial space of the testis as early as embryonic day 12.5 and thereafter increase in number during the fetal stage. We previously established a transgenic mouse line in which FLCs are labeled with EGFP and demonstrated that the EGFP-labeled FLCs were present even in adult testes. However, the characteristics of FLCs during postnatal stages remained unclear. In the present study, a comparison of the transcriptomes of FLCs from prenatal and postnatal testes and of ALCs from adult testes revealed that FLCs gradually alter their characteristics across developmental stages and come to roughly resemble ALCs. Many cholesterogenic genes simultaneously expressed a unique alternation pattern, while many oxidative phosphorylation and beta-oxidation (both mitochondrial functions) genes showed a different unique pattern. These metabolic gene expression alterations might be triggered by milieu changes, such as nutrient and oxygen supply, from the prenatal to the postnatal period. (C) 2016 S.Karger AG, Basel