Use of cross-reactive serological assays for detecting novel pathogens in wildlife: Assessing an appropriate cutoff for henipavirus assays in African bats
Use of cross-reactive serological assays for detecting novel pathogens in wildlife: Assessing an appropriate cutoff for henipavirus assays in African bats
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DOI：
10.1016/j.jviromet.2013.06.030
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发表时间：
2013-11-01
影响因子：
3.1
通讯作者：
Wood, James L. N.
中科院分区：
文献类型：
--
作者：
Peel, Alison J.;McKinley, Trevelyan J.;Wood, James L. N.
在病原体本身分离之前，通常根据血清学检测结果识别或怀疑新病原体的存储宿主。因此，在开发出特定的诊断分析方法之前，可以在其原始的、经过验证的范围之外使用血清学检测，以推断宿主人群中的血清抗体水平。在野生动物疾病研究中尤其如此。由于缺乏阳性和阴性对照样本和金标准诊断分析，为能够区分血清阴性和血清阳性个体的分析确定适当的阈值或‘截止’带来了挑战。在这里，探索了多种方法来确定用于检测果蝠血浆中HeipaVirus抗体结合的多重微球试验的合适的截止范围。这些方法包括计算“阴性”对照化验值的倍数，接收者操作特征曲线分析，以及贝叶斯混合模型，以评估对不同年龄段的血清阳性和血清阴性个体进行分类的分析输出的分布。对于任何诊断分析，必须根据研究的目的选择最合适的截止测定方法和值。这项研究是作为其他没有参考样品和以前描述的分析的例子而提出的。(C)2013年提交人。爱思唯尔出版，版权所有。
Reservoir hosts of novel pathogens are often identified or suspected as such on the basis of serological assay results, prior to the isolation of the pathogen itself. Serological assays might therefore be used outside of their original, validated scope in order to infer seroprevalences in reservoir host populations, until such time that specific diagnostic assays can be developed. This is particularly the case in wildlife disease research. The absence of positive and negative control samples and gold standard diagnostic assays presents challenges in determining an appropriate threshold, or 'cutoff', for the assay that enables differentiation between seronegative and seropositive individuals. Here, multiple methods were explored to determine an appropriate cutoff for a multiplexed microsphere assay that is used to detect henipavirus antibody binding in fruit bat plasma. These methods included calculating multiples of `negative' control assay values, receiver operating characteristic curve analyses, and Bayesian mixture models to assess the distribution of assay outputs for classifying seropositive and seronegative individuals within different age classes. As for any diagnostic assay, the most appropriate cutoff determination method and value selected must be made according to the aims of the study. This study is presented as an example for others where reference samples, and assays that have been characterised previously, are absent. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.