Synthesis and Degradation of Polyphosphate: Isotope Effects in Enzyme- and Bacteria-Catalyzed Reactions
Synthesis and Degradation of Polyphosphate: Isotope Effects in Enzyme- and Bacteria-Catalyzed Reactions
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DOI：
10.1021/acsearthspacechem.0c00230
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发表时间：
2020-12-17
影响因子：
3.4
通讯作者：
Jaisi, Deb
中科院分区：
文献类型：
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作者：
Bai, Yuge;Stout, Lisa;Jaisi, Deb
多聚磷酸（poly-P）是一种通过高能磷酸酐键连接的磷酸基团链，在原核细胞中起着重要的调控作用。细菌合成和降解聚磷的同位素效应可能为了解聚磷在环境磷循环中的作用提供重要的线索。本研究分别采用无细胞酶解液和细菌细胞对聚磷酰胺进行酶解（大肠杆菌JM 103和恶臭假单胞菌KT 2440），并分析了磷酸盐氧同位素比值（δ O-18（P）），酶活性，和动力学的正磷酸盐（P-i）和聚-P。细胞-游离酶反应结果表明，酸性和碱性磷酸酶都能够催化聚磷降解，尽管效率不同，>70，
Polyphosphate (poly-P) is a chain of phosphate moieties linked through high-energy phosphoanhydride bond, and it plays an important role in regulatory functions in prokaryotic cells. Isotope effects of bacterial synthesis and degradation of poly-P may provide important insights into the roles of poly-P in environmental phosphorus (P) cycling. In this research, we investigated the enzymatic degradation of poly-P by cell-free enzyme solutions and bacterial cells (Escherichia coli JM103 and Pseudomonas putida KT2440) and analyzed phosphate oxygen isotope ratios (delta O-18(P)), enzyme activity, and dynamics of orthophosphate (P-i) and poly-P. Cell-free enzyme reaction results show that both acid and alkaline phosphatase enzymes are capable of catalyzing poly-P degradation, albeit with different efficiencies of >70 and