High-speed particle separation and steric inversion in thin flow field-flow fractionation channels
High-speed particle separation and steric inversion in thin flow field-flow fractionation channels
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DOI：
10.1016/0021-9673(96)00288-9
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发表时间：
1996-10-04
影响因子：
4.1
通讯作者：
Giddings, JC
中科院分区：
文献类型：
--
作者：
Jensen, KD;Williams, SKR;Giddings, JC
在高通道弓率（高达类似于 47 ml/min）和错流速率（高达类似于 11 ml/min）下检查了薄（低至 71 μm 厚）流场流分级（流 FFF）通道的性能。观察到高水平的保留，表明良好的性能特征。支持这一预期的是，四种尺寸（0.04-0.30μm）的聚苯乙烯乳胶微珠在 3 分钟内进行了基线分离。尽管如此，板高度分析表明性能仍然低于理论预期。在相同的系统中观察到较大乳胶珠的快速空间超层分离。此外，结果表明，空间反转直径向下移动至约0.23μm，从而扩大了该FFF模式适用的尺寸范围。使用窄胶乳标准观察到的空间反转现象与多分散聚氯乙烯胶乳的空间反转现象一致。
The performance of thin (down to 71 mu m thick) flow field-flow fractionation (flow FFF) channels was examined at both high channel Bow-rates (up to similar to 47 ml/min) and cross flow-rates (up to similar to 11 ml/min). High levels of retention were observed, suggestive of good performance characteristics. Supporting this expectation, four sizes (0.04-0.30 mu m) of polystyrene latex microbeads were baseline separated in under 3 min. Nonetheless, a plate height analysis showed that performance was still less than theoretically expected.Fast steric-hyperlayer separations of larger latex beads were observed in the same systems. Furthermore, it was shown that the steric inversion diameter was shifted down to similar to 0.23 mu m, thus expanding the size range to which this FFF mode is applicable. The steric inversion phenomenon observed using narrow latex standards was shown to be consistent with that found for a polydisperse polyvinylchloride latex.