Kefir extracts suppress in vitro proliferation of estrogen-dependent human breast cancer cells but not normal mammary epithelial cells
Kefir extracts suppress in vitro proliferation of estrogen-dependent human breast cancer cells but not normal mammary epithelial cells
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DOI：
10.1089/jmf.2006.236
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发表时间：
2007-09-01
影响因子：
2.4
通讯作者：
Kubow, Stan
中科院分区：
文献类型：
--
作者：
Chen, Chujian;Chan, Hing Man;Kubow, Stan
在动物研究中，通过摄入克非尔已经证明了抗肿瘤作用，克非尔是一种传统的发酵乳制品，据信起源于俄罗斯高加索山脉。本研究分别以0.31%、0.63%、1.25%、2.5%、5%和10% （vol/vol）为剂量，研究了开菲尔、酸奶和巴氏牛奶提取物对人乳腺癌细胞（MCF-7）和正常人乳腺上皮细胞（HMECs）的抗增殖作用。培养6天后，开菲尔发酵乳提取物以剂量依赖的方式抑制MCF-7细胞的生长，在低至0.63%的浓度下表现出29%的增殖抑制作用，而酸奶提取物仅在2.5%的剂量下才开始表现出剂量依赖的抗增殖作用。此外，在2.5%剂量下，开菲尔提取物使MCF-7细胞数量减少56%，而酸奶提取物仅使MCF-7细胞增殖减少14%。开菲尔提取物对hmec无抑制作用，而酸奶提取物在5%和10%剂量下对hmec有抑制作用。未发酵乳提取物对MCF-7细胞和HMECs的增殖有促进作用，浓度在0.31%以上。肽含量和毛细管电泳分析表明，与牛奶或酸奶相比，开菲尔介导的牛奶发酵导致了肽浓度的增加和肽谱的变化。目前的研究结果表明，开非尔提取物含有特异性抑制人类乳腺癌细胞生长的成分，这可能最终有助于预防或治疗乳腺癌。
Anti-tumorigenic effects have been demonstrated in animal studies from the intake of kefir, a traditional fermented milk product believed to originate from the Caucasian mountains of Russia. In the present study, the antiproliferative effects of extracts of kefir, yogurt, and pasteurized cow's milk on human mammary cancer cells (MCF-7) and normal human mammary epithelial cells (HMECs) was investigated at doses of 0.31%, 0.63%, 1.25%, 2.5%, 5%, and 10% (vol/vol). After 6 days of culture, extracts of kefir-fermented milk depressed MCF-7 cell growth in a dose-dependent manner, showing 29% inhibition of proliferation at a concentration as low as 0.63%, whereas yogurt extracts began to show dose-dependent antiproliferative effects only at the 2.5% dose. Moreover, at the 2.5% dose, kefir extracts decreased the MCF-7 cell numbers by 56%, while yogurt extracts decreased MCF-7 cell proliferation by only 14%. No antiproliferative effects of kefir extracts were observed in the HMECs, while the yogurt extracts exerted antiproliferative effects on HMECs at the 5% and 10% doses. Unfermented milk extracts stimulated proliferation of MCF-7 cells and HMECs at concentrations above 0.31%. Peptide content and capillary electrophoresis analyses showed that kefir-mediated milk fermentation led to an increase in peptide concentrations and a change in peptide profiles relative to milk or yogurt. The present findings suggest that kefir extracts contain constituents that specifically inhibit the growth of human breast cancer cells, which might eventually be useful in the prevention or treatment of breast cancer.