Anthocyanin Accumulation, Antioxidant Ability and Stability, and a Transcriptional Analysis of Anthocyanin Biosynthesis in Purple Heading Chinese Cabbage (Brassica rapa L. ssp pekinensis)
Anthocyanin Accumulation, Antioxidant Ability and Stability, and a Transcriptional Analysis of Anthocyanin Biosynthesis in Purple Heading Chinese Cabbage (Brassica rapa L. ssp pekinensis)
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DOI：
10.1021/acs.jafc.5b04674
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发表时间：
2016-01-13
影响因子：
6.1
通讯作者：
Zhang, Lugang
中科院分区：
文献类型：
--
作者：
He, Qiong;Zhang, Zhanfeng;Zhang, Lugang
结球白菜（Brassica rapa L.）SSP. pekinensis）是亚洲国家重要的食用蔬菜，其头叶可食用。新的紫色花色苷富集纯系（11 S91）选育成功，花色苷主要分布在叶表皮下的2-3个细胞层中，而角果和茎中只积累了一个细胞层。11 S91的花色苷在pH低于3.0和温度低于45 ℃时更稳定。11 S91的总抗氧化能力与花色苷含量呈极显著正相关。共分离鉴定了32种花色苷，其中70%为糖基化和酰化花青素。存在的四种主要花色素苷是矢车菊素-3-槐糖苷（对香豆酰基）-5-葡萄糖苷（丙二酰基）、矢车菊素-3-槐糖苷（阿魏酰基）-5-葡萄糖苷（丙二酰基）、矢车菊素-3-槐糖苷（芥子基-对香豆酰基）-5-葡萄糖苷（丙二酰基）和矢车菊素-3-槐糖苷-（芥子基-阿魏酰基）-5-葡萄糖苷（丙二酰基）。根据11 S91及其亲本花色素苷生物合成基因的表达和花色素苷组分谱，调控基因BrMYB 2和BrTT 8可能是花色素苷生物合成的调控基因，但其它因素可能控制初级花色素苷的合成和分布。
Heading Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a significant dietary vegetable for its edible heading leaves in Asia countries. The new purple anthocyanin-rich pure line (11S91) was successfully bred, and the anthocyanins were mainly distributed in 2-3 cell layers beneath the leaf epidermis, whereas siliques and stems accumulated only a cell layer of anthocyanins. The anthocyanins of 11S91 were more stable at pHs below 3.0 and temperatures below 45 degrees C. The total antioxidant ability was highly positive correlated with the anthocyanin content in 11S91. Thirty-two anthocyanins were separated and identified, and 70% of them were glycosylated and acylated cyanidins. The four major anthocyanins present were cyanidin-3-sophoroside(p-coumaroyl)-5-glucoside(malonyl), cyanidin-3-sophoroside(ferulyl)-5-glucoside(malonyl), cyanidin-3-sophoroside(sinapyl-p-coumaroyl)-5-glucoside(malonyl), and cyanidin-3-sophoroside-(sinapyl-ferulyl)-5-glucoside (malonyl). According to the expression of biosynthetic genes and the component profile of anthocyanins in 11S91 and its parents, regulatory genes BrMYB2 and BrTT8 probably activate the anthocyanin biosynthesis but other factors may govern the primary anthocyanins and the distribution.