Metabolomics of bacterial-fungal pairwise interactions reveal conserved molecular mechanisms.
Metabolomics of bacterial-fungal pairwise interactions reveal conserved molecular mechanisms.
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细菌-真菌配对相互作用的代谢组学揭示了保守的分子机制。
DOI：
10.1039/d3an00408b
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Sanchez,LauraM
中科院分区：
文献类型：
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作者：
Luu,GordonT;Little,JessicaC;Pierce,EmilyC;Morin,Manon;Ertekin,CelineA;Wolfe,BenjaminE;Baars,Oliver;Dutton,RachelJ;Sanchez,LauraM
细菌-真菌相互作用(BFI)可以塑造微生物群落的结构，但介导这些BFI的小分子往往被研究得很少。我们探索了微生物培养和细菌-真菌共培养的化学提取方案的各种优化步骤，液-质联用(LC-MS/MS)显示代谢谱主要由真菌衍生的特征组成，表明真菌是BFIS中小分子的关键贡献者。LC-ICP-MS和基于MS/MS的数据库搜索发现，这些提取物中存在一些已知的真菌专化代谢物和结构相关的类似物，包括铁载体，如去铁色素、去铁原和棕榈酰辅原。在这些类似物中，一个新的具有末端羧酸基序的辅原类似物被从Scopulariopsis sp.JB370是一种常见的干酪皮真菌，其结构经MS/MS鉴定。根据这些发现，丝状真菌物种似乎能够产生具有潜在不同生物学作用的多个铁载体(即不同形式的铁具有不同的亲和力)。这些发现突出表明，真菌物种通过产生丰富的专门代谢物，是微生物群的重要贡献者，阐明它们在复杂群落中的作用应继续是优先事项。
Bacterial–fungal interactions (BFIs) can shape the structure of microbial communities, but the small molecules mediating these BFIs are often understudied. We explored various optimization steps for our microbial culture and chemical extraction protocols for bacterial–fungal co-cultures, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that metabolomic profiles are mainly comprised of fungi derived features, indicating that fungi are the key contributors to small molecules in BFIs. LC-inductively coupled plasma MS (LC-ICP-MS) and MS/MS based dereplication using database searching revealed the presence of several known fungal specialized metabolites and structurally related analogues in these extracts, including siderophores such as desferrichrome, desferricoprogen, and palmitoylcoprogen. Among these analogues, a novel putative coprogen analogue possessing a terminal carboxylic acid motif was identified from Scopulariopsis sp. JB370, a common cheese rind fungus, and its structure was elucidated via MS/MS fragmentation. Based on these findings, filamentous fungal species appear to be capable of producing multiple siderophores with potentially different biological roles (i.e. various affinities for different forms of iron). These findings highlight that fungal species are important contributors to microbiomes via their production of abundant specialized metabolites and that elucidating their role in complex communities should continue to be a priority.