Predominance of Ehrlichia ewingii in Missouri dogs
Predominance of Ehrlichia ewingii in Missouri dogs
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DOI：
10.1128/jcm.41.10.4617-4622.2003
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发表时间：
2003-10-01
影响因子：
9.4
通讯作者：
Storch, GA
中科院分区：
文献类型：
--
作者：
Liddell, AM;Stockham, SL;Storch, GA
为了调查在密苏里犬中存在的埃利希体的种类分布，我们对78只疑似患有急性埃利希体病的犬和10只健康犬进行了测试。对每只狗的血液进行宽范围16S rRNA基因PCR检测，检测已知的致病性埃利希体和无形体。采用种特异性PCR法和核苷酸测序法确定物种。以沙菲埃立体为抗原底物，采用间接免疫荧光法检测埃立体抗体。在有症状犬中检测到埃利希体或无原体DNA的有20只（26%），在无症状犬中检测到2只（20%）。ewingii伊蚊20只（91%），chaffeensis 1只（5%）。在一只犬身上检测到嗜吞噬细胞无原体DNA，从该犬血液中扩增出的16S rRNA基因和groESL操纵子区域序列与已发表的该生物序列相匹配。21只pcr阳性犬中14只（67%）和64只pcr阴性犬中12只（19%）检测到沙非埃希菌抗体。结合PCR和血清学结果表明，85只可评估的狗中有33只（39%）有过去或现在感染埃利希体的证据。我们得出结论，伊温伊氏伊蚊是本次调查中密苏里州地区犬埃利希体病的主要病原。犬伊氏体是公认的犬埃利希体病病原，未在任何动物中检测到。在无症状犬中发现的伊温伊氏埃利希菌表明犬可能是该埃利希菌种的宿主。
To investigate the species distribution of Ehrlichia present in Missouri dogs, we tested 78 dogs suspected of having acute ehrlichiosis and 10 healthy dogs. Blood from each dog was screened with a broad-range 16S rRNA gene PCR assay that detects known pathogenic species of Ehrlichia and Anaplasma. The species was determined by using species-specific PCR assays and nucleotide sequencing. Ehrlichia antibody testing was performed by using an indirect immunofluorescence assay with Ehrlichia chaffeensis as the antigenic substrate. The broad-range assay detected Ehrlichia or Anaplasma DNA in 20 (26%) of the symptomatic dogs and 2 (20%) of the asymptomatic dogs. E. ewingii accounted for 20 (91%), and E. chaffeensis accounted for 1 (5%) of the positives. Anaplasma phagocytophilum DNA was detected in one dog, and the sequences of regions of the 16S rRNA gene and the groESL operon amplified from the blood of this dog matched the published sequences of this organism. Antibodies reactive with E. chaffeensis were detected in 14 (67%) of the 21 PCR-positive dogs and in 12 (19%) of the 64 PCR-negative dogs. Combining the results of PCR and serology indicated that 33 (39%) of 85 evaluable dogs had evidence of past or current Ehrlichia infection. We conclude that E. ewingii is the predominant etiologic agent of canine ehrlichiosis in the areas of Missouri included in this survey. E. canis, a widely recognized agent of canine ehrlichiosis, was not detected in any animal. The finding of E. ewingii in asymptomatic dogs suggests that dogs could be a reservoir for this Ehrlichia species.