Differentiating sulfopeptide and phosphopeptide ions via resonant infrared photodissociation.
Differentiating sulfopeptide and phosphopeptide ions via resonant infrared photodissociation.
复制标题
通过共振红外光解区分硫肽和磷酸肽离子。
作者：
Amanda L. Patrick;Corey N. Stedwell;N. Polfer
翻译后修饰的硫酸化和磷酸化对质谱分析提出了特殊的挑战，因为它们的等压性质和在气相中的不稳定性，因为这两种类型的多肽在碰撞激活时通过相似的通道解离。在这里，我们提出了基于诊断硫酸盐和磷酸羟基伸展的共振红外光解，作为在质谱学平台框架内区分硫酸盐和磷酸化肽的一种手段。该方法被证明用于许多含有酪氨酸的多肽，从二肽(YG、PYG和SYG)到三肽(GyR、GPYR和GsYR)，到更具生物相关性的脑啡肽(YGGFL、pYGGFL和sYGGFL)。在所有情况下，硫酸盐羟基伸展的诊断范围被确定为3580-3600厘米(-1)，因此可以与其他特征氢伸展区分开来，例如羧酸羟基伸展、醇羟基伸展和磷酸羟基伸展。
The post-translational modifications sulfation and phosphorylation pose special challenges to mass spectral analysis due to their isobaric nature and their lability in the gas phase, as both types of peptides dissociate through similar channels upon collisional activation. Here, we present resonant infrared photodissociation based on diagnostic sulfate and phosphate OH stretches, as a means to differentiate sulfated from phosphorylated peptides within the framework of a mass spectrometry platform. The approach is demonstrated for a number of tyrosine-containing peptides, ranging from dipeptides (YG, pYG, and sYG) over tripeptides (GYR, GpYR, and GsYR), to more biologically relevant enkephalin peptides (YGGFL, pYGGFL, and sYGGFL). In all cases, the diagnostic ranges for sulfate OH stretches are established as 3580-3600 cm(-1) and can thus be distinguished from other characteristic hydrogen stretches, such as carboxylic acid OH, alcohol OH, and phosphate OH stretches.
IRMPD 光谱显示 AGG 形成恶唑酮 b2 离子。
影响因子：
15
作者：
Yoon,SungHwan;Chamot-Rooke,Julia;Perkins,BrittanyR;Hilderbrand,AmyE;Poutsma,JohnC;Wysocki,VickiH
通讯作者：
Wysocki,VickiH
影响因子：
62.1
作者：
Zhang, Yaoyang;Fonslow, Bryan R.;Shan, Bing;Baek, Moon-Chang;Yates, John R., III
通讯作者：
Yates, John R., III
影响因子：
6.1
作者：
Dass, C;Mahalakshmi, P
通讯作者：
Mahalakshmi, P