Development and Characterization of Microfluidic Devices and Systems for Magnetic Bead-Based Biochemical Detection
Development and Characterization of Microfluidic Devices and Systems for Magnetic Bead-Based Biochemical Detection
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DOI：
10.1023/a:1011490627871
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发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Ahn, Chong H.
中科院分区：
文献类型：
--
作者：
Choi, Jin-Woo;Oh, Kwang W.;Ahn, Chong H.
本文介绍了一个通用的微流控系统的磁珠为基础的生化检测的发展和表征。微流控和电化学检测设备，如微阀，流量传感器，生物过滤器和免疫传感器已成功开发，并在这项工作中单独表征。磁驱动微阀，脉冲模式微流量传感器，磁性颗粒分离器作为生物过滤器，和电化学免疫传感器已分别制造和测试。将所制备的微流控元件表面贴装在微流控母板上，实现了微流控生化检测系统的全集成，并采用生物磁珠法实现了对目标生物分子的采样和操作。磁珠被用作抗体的基底和靶抗原的载体用于基于磁珠的免疫测定，其被选择作为通用微流控生化检测系统的概念验证。从这项工作中获得的微流控和电化学免疫传感实验结果表明，完整的微流控子系统的生化传感能力适合于生物分子的便携式生化检测。可以通过用适当的生物受体替代抗体/抗原而扩展到通用的生物分子检测和分析系统，用于DNA分析和/或高通量蛋白质分析的试剂如DNA片段或寡核苷酸。
This paper presents the development and characterization of a generic microfluidic system for magnetic bead-based biochemical detection. Microfluidic and electrochemical detection devices such as microvalves, flow sensors, biofilters, and immunosensors have been successfully developed and individually characterized in this work. Magnetically driven microvalves, pulsed-mode microflow sensors, magnetic particle separators as biofilters, and electrochemical immunosensors have been separately fabricated and tested. The fabricated microfluidic components have been surface-mounted on the microfluidic motherboard for fully integrated microfluidic biochemical detection system.A magnetic bio-bead approach has been adopted for both sampling and manipulating target biological molecules. Magnetic beads were used as both substrate of antibodies and carriers of target antigens for magnetic bead-based immunoassay, which was chosen as a proof-of-concept for the generic microfluidic biochemical detection system. The microfluidic and electrochemical immunosensing experiment results obtained from this work have shown that the biochemical sensing capability of the complete microfluidic subsystem is suitable for portable biochemical detection of bio-molecules.The methodology and system, which has been developed in this work, can be extended to generic bio-molecule detection and analysis systems by replacing antibody/antigen with appropriate bio receptors/reagents such as DNA fragments or oligonucleotides for application towards DNA analysis and/or high throughput protein analysis.