Enhancement of misonidazole chemopotentiation by mild hyperthermia (41 degrees C) in vitro and selective enhancement in vivo.
Enhancement of misonidazole chemopotentiation by mild hyperthermia (41 degrees C) in vitro and selective enhancement in vivo.
复制标题
体外轻度热疗（41℃）增强米索硝唑化学增效作用，体内选择性增强。
DOI：
10.1080/09553008714551481
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Tanner,MA
中科院分区：
文献类型：
--
作者：
Mulcahy,RT;Gipp,JJ;Tanner,MA
Experiments were designed to test the hypothesis that mild heat treatment would selectively increase misonidazole (MISO) chemopotentiation of CCNU toxicity in hypoxic versus aerobic cellsin vitroand in tumoursin vivovia an augmentation of nitroreduction. EMT-6 cells were exposed to CCNU ± 1·0 mmMISO under aerobic or hypoxic conditions for 4 h either at a constant 37°C or at 41°C for the first hour followed by 37°C for the remaining 3 h. Chemopotentiation was not observed under aerobic conditions and heat treatment did not modify CCNU toxicity. Co-incubation with MISO and CCNU under hypoxic conditions resulted in enhanced toxicity (i.e. chemopotentiation) with either incubation protocol; however, the magnitude of the enhancement was significantly larger (P< 0·025) when 41°C incubation was included. Systemic heat treatment produced a similar enhancement of chemopotentiation in KHT tumours in C3H/HeN mice treated with MISO (0·5 mg g−1) and whole body hyperthermia (41°C, 1 h) prior to administration of CCNU (15 mg kg−1). Heating had no effect on CCNU response but doubled the median growth delay produced by the CCNU-MISO combination. Heat treatment did not enhance myelosuppression of the combination. Both thein vitroandin vivodata indicate that mild hyperthermia can selectively enhance the magnitude of MISO chemopotentiation.
