VNTR (variable number of tandem repeats) markers show loss of chromosome 17p sequences in human colorectal carcinomas.
VNTR (variable number of tandem repeats) markers show loss of chromosome 17p sequences in human colorectal carcinomas.
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VNTR（可变数量串联重复）标记显示人类结直肠癌中染色体 17p 序列丢失。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
R. White
中科院分区：
文献类型：
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作者：
R. Lothe;Y. Nakamura;S. Woodward;T. Gedde;R. White
对40例结直肠癌患者进行了53个常染色体位点的限制性片段长度多态性杂合性筛查。比较了体质与肿瘤/息肉组织中的DNA模式。超过一半的测试标记是VNTR（可变数目的串联重复序列）类型，给予患者面板更高的信息含量，因为个体的频率杂合的特定标记增加。在40%的来自染色体17 p基因座上的组成型杂合患者的肿瘤中揭示了等位基因的丢失，所述杂合患者由标记YNH 37和YNZ 22鉴定。在其他常染色体上也检测到类似的丢失，但频率明显较低。我们的研究结果表明，17 p等位基因的半/纯合性在结直肠癌的一个主要子集的发展中发挥了作用。关于其他常染色体基因座的类似观察结果可能被解释为这些肿瘤中的随机丢失，或者它们可能指示对结肠癌的次要临床亚类重要的基因座。
Restriction fragment length polymorphisms at 53 autosomal loci were screened for heterozygosity in 40 colorectal cancer patients. The DNA pattern in constitutional versus tumor/polyp tissue was compared. More than half of the markers tested were of the VNTR (variable number of tandem repeats) type, giving the patient panel a higher informational content, since the frequency of individuals heterozygous for a particular marker is increased. Loss of alleles was revealed in 40% of the tumors from constitutionally heterozygous patients at the chromosome 17p loci, identified by the markers YNH37 and YNZ22. Similar losses were also detected on other autosomes, but at a significantly lower frequency. Our results suggest that hemi/homozygosity of 17p alleles plays a role in the development of a major subset of colorectal carcinomas. Similar observations regarding other autosomal loci may be interpreted as random losses in these tumors, or they may indicate loci important to minor clinical subclasses of colon carcinomas.