Early cellular development induced by ecdysteroid in sex-specific wing degeneration of the wingless female winter moth
Early cellular development induced by ecdysteroid in sex-specific wing degeneration of the wingless female winter moth
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蜕皮类固醇诱导无翅雌性冬蛾性别特异性翅膀退化的早期细胞发育
DOI：
10.1007/s00441-021-03540-6
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发表时间：
2021
影响因子：
3.6
通讯作者：
Kamito Takehiko
中科院分区：
文献类型：
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作者：
Niitsu Shuhei;Kamito Takehiko
越冬蛾Nyssiodes lefuarius在翅膀形式上表现出惊人的性别二型性；雄性有正常大小的功能性翅膀，而雌性没有翅膀。我们先前发现，类固醇激素20-羟基蜕皮酮(20E)只在雌性蛹翅上皮细胞中引发大量的程序性细胞死亡(PCD)；然而，在蛹-成虫变态过程中，蛹翅的早期性征发育何时以及如何开始仍然是未知的。为了阐明早期性征发育和细胞死亡的详细形态变化和机制，我们研究了20E对雌雄幼虫蛹翅上皮早期超微结构和组织学变化的影响。在成体分化开始之前，在超微结构水平上没有观察到两性上皮细胞的形态差异。化蛹开始后15天，雌蛾蛹内注射5.4g 20E，雌虫在注射20E后第2天开始将翅上皮从蛹的角质层中抽离。虽然翅组织变性程度尚不明显，但雌鼠在注射20E后3d可见凋亡体样结构和自噬小体，雄鼠于注射后4d开始发育鳞片前体细胞。我们的结果提示：(1)注射20E可引起翅上皮细胞内细胞器分布模式的性二态改变，(2)雌翅上皮细胞质内线粒体形状异常可能参与了翅组织退行性变过程中的PCD。
The winter moth,Nyssiodes lefuarius, exhibits striking sexual dimorphism in wing form; males have functional wings of normal size, whereas females lack wings. We previously found that the steroid hormone 20-hydroxyecdysone (20E) triggered massive programmed cell death (PCD) only in the female pupal wing epithelium; however, when and how early sexual trait development of the pupal wings is initiated during pupal-adult metamorphosis remains obscure. To clarify the detailed morphological changes and mechanisms underlying early sexual trait development and cell death, we examined the effects of 20E on early ultrastructural and histological changes in the pupal wing epithelium of both sexes. Before the onset of adult differentiation, no morphological differences were observed in the epithelial cells of both sexes at an ultrastructural level. When 5.4 µg of 20E was injected into pupae of both sexes at 15 days after the onset of pupation, retraction of the wing epithelium from the pupal cuticle was initiated at day 2 after 20E injection in both sexes. Although overt degeneration of wing tissue was not still obvious, apoptotic body-like structures and auto-phagosomes were visible at day 3 after 20E injection in females, whereas development of scale precursor cells started on day 4 after injection in males. Our results suggest that (1) the injection of 20E induced sexually dimorphic changes in the pattern of organelle distribution in wing epithelial cells, and (2) abnormally shaped mitochondria in the cytoplasm of the female wing epithelium might be involved in the PCD that occurs during wing tissue degeneration.