The role of intercellular contacts in the activation of B lymphocytes by anti-immunoglobulin antibodies.
The role of intercellular contacts in the activation of B lymphocytes by anti-immunoglobulin antibodies.
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细胞间接触在抗免疫球蛋白抗体激活 B 淋巴细胞中的作用。
DOI：
10.4049/jimmunol.134.5.2827
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发表时间：
1985
影响因子：
4.4
通讯作者：
M. Teodorescu
中科院分区：
文献类型：
--
作者：
H. Spieker;K. Hagen;M. Teodorescu
已经提出抗Ig抗体以类似于抗原的方式激活B细胞，即，它通过交联和聚集一个细胞表面上的IG受体来传递信号。然而，尚未考虑可相互传递信号的不同B细胞的交联。因此，我们通过使用固体琼脂糖培养基检测了防止细胞接触对兔B细胞对抗Ig同种异型抗体的应答的影响。首先，我们检查了14 C-尿苷掺入液体培养基和液体或固体琼脂糖在培养物中刺激抗Ig抗体（Ab）。在液体琼脂糖或液体培养基中的反应很高，但在培养开始时含琼脂糖的培养基固化时不存在。如果琼脂糖在开始后6小时固化，则获得良好的响应。此外，如果在固化含琼脂糖的培养基之前开始沉淀细胞，也可获得良好的反应。当T细胞被Con A激活时，获得类似的结果。为了检查B细胞是否需要与其他B细胞或非B细胞接触，我们在不存在巨噬细胞或T细胞的情况下检查了它们对抗Ig Ab的应答，发现来自淋巴器官或来自胸导管的纯化B细胞在不存在T细胞和/或巨噬细胞的情况下应答良好。当细胞在琼脂中以接近10（8）个细胞/ml的“局部”浓度培养时，也不存在反应。此外，抗Ig刺激细胞的浓缩上清液不增加固体琼脂糖中抗Ig的反应。这最后两个观察结果表明，固体琼脂糖中缺乏反应不是由于缺乏扩散因子或缺乏饲养细胞。因此，由于对抗Ig或Con A有反应的培养物与没有反应的培养物之间的唯一区别是细胞之间的距离，我们得出结论，B细胞或T细胞之间的接触对于它们各自的有丝分裂原激活它们是必不可少的。我们推测抗Ig Ab或抗原交联B细胞，然后它们相互提供激活信号或其中一种激活信号。
It has been proposed that anti-Ig antibody activates B cells in a way analogous to the antigens, i.e., it delivers its signal by cross-linking and clustering the Ig receptors on the surface of one cell. However, the cross-linking of different B cells which may deliver to each other a signal has not been considered. Thus, we examined the effect of preventing cell contacts on the response of rabbit B cells to anti-Ig allotype antibody by using a solid agarose medium. First, we examined the 14C-uridine incorporation in liquid medium and in liquid or solid agarose in cultures stimulated with anti-Ig antibody (Ab). The response was high in liquid agarose or liquid medium but was absent when the agarose-containing medium was solidified at the start of the cultures. If the agarose was solidified 6 hr after the start, a good response was obtained. Moreover, if the cells were sedimented at the start before solidifying the agarose-containing medium, a good response was also obtained. Similar results were obtained when T cells were activated by Con A. To examine whether B cells require contacts with other B cells or with non-B cells, we examined their response to anti-Ig Ab in the absence of macrophages or T cells, and found that purified B cells from lymphoid organs or from thoracic duct responded well in the absence of T cells and/or macrophages. The response was also absent when the cells were cultured in agar at a "local" concentration close to 10(8) cell/ml. Also, concentrated supernatants of anti-Ig-stimulated cells did not increase the response to anti-Ig in solid agarose. These two last observations suggest that the lack of response in solid agarose is not due to a lack of diffusible factors or to a lack of feeder cells. Therefore, because the only difference between the cultures that responded to anti-Ig or Con A and those that did not was the distance between the cells, we concluded that the contact between B cells or between T cells is essential to their activation by their respective mitogens. We speculate that the anti-Ig Ab or the antigen cross-links B cells, which then provide each other with the activating signal or with one of the activating signals.