A CIN-like TCP transcription factor (LsTCP4) having retrotransposon insertion associates with a shift from Salinas type to Empire type in crisphead lettuce (Lactuca sativa L.)
A CIN-like TCP transcription factor (LsTCP4) having retrotransposon insertion associates with a shift from Salinas type to Empire type in crisphead lettuce (Lactuca sativa L.)
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DOI：
10.1038/s41438-020-0241-4
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发表时间：
2020-02-01
影响因子：
8.7
通讯作者：
Uno, Yuichi
中科院分区：
文献类型：
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作者：
Seki, Kousuke;Komatsu, Kenji;Uno, Yuichi
为了改良脆头莴苣（Lactuca sativa L.）在高温生长条件下，我们调查了多个品种的这些性状之间的相关性，并对其致病基因进行了遗传定位。在帝国型（锯齿叶）和萨利纳斯型（波形叶）品种的田间栽培试验中，帝国型品种表现出增加的烧心感和晚抽薹与萨利纳斯型品种相比。我们尝试通过ddRAD-seq使用衍生自'VI 185'（Empire类型）和'ShinanoGreen'（Salinas类型）之间的杂交的F2群体对叶片形状和抽薹时间进行遗传作图。这些分析表明，这两个性状是由一个单一的基因座在LG 5。利用该位点的紧密连锁标记（LG5_v8_252.743Mbp）对51个生菜品种进行基因分型，结果表明该位点的基因型与锯齿叶表型相关。通过进一步的精细定位和转录组分析，编码推定的CIN样TCP转录因子的基因被确定为该位点的候选基因，并被命名为LsTCP 4。在‘VI 185’的等位基因中发现了反转录转座因子的插入，其在叶片中的转录水平低于‘ShinanoGreen’。由于“VI 185”中的叶表皮细胞的形状与拟南芥TCP家族突变体中的叶表皮细胞的形状相似，因此叶形状表型可能是由LsTCP 4表达减少引起的。此外，由于已知TCP家族蛋白也通过与A.在拟南芥中，LsTCP 4极有可能对莴苣的叶片形状和抽薹时间都具有多效性。
To improve several agronomic traits in crisphead lettuce (Lactuca sativa L.) under high-temperature growth conditions, we investigated the correlation among those traits in multiple cultivars and performed genetic mapping of their causal genes. In a field cultivation test of Empire type (serrated leaf) and Salinas type (wavy leaf) cultivars, Empire type cultivars showed increased tipburn susceptibility and late bolting compared with Salinas type cultivars. We attempted genetic mapping of leaf shape and bolting time by ddRAD-seq using the F2 population derived from a cross between 'VI185' (Empire type) and 'ShinanoGreen' (Salinas type). These analyses suggested that both traits are controlled by a single locus in LG5. Genotyping of 51 commercial lettuce cultivars with a tightly linked marker (LG5_v8_252.743Mbp) at this locus showed an association between its genotype and the serrated leaf phenotype. By further fine mapping and transcriptome analysis, a gene encoding putative CIN-like TCP transcription factor was determined to be a candidate gene at this locus and was designated as LsTCP4. An insertion of retrotransposable element was found in the allele of 'VI185', and its transcript level in the leaves was lower than that in 'ShinanoGreen'. Because shapes of leaf epidermal cells in 'VI185' were similar to those in the TCP family mutant of Arabidopsis thaliana, the leaf shape phenotype was likely caused by reduced expression of LsTCP4. Furthermore, because it is known that the TCP family protein also controls flowering time via interaction with FT in A. thaliana, it was highly possible that LsTCP4 gave pleiotropic effects on both leaf shape and bolting time in lettuce.