Anxiety-like behavior and neuroendocrine changes in offspring resulting from gestational post-traumatic stress disorder
Anxiety-like behavior and neuroendocrine changes in offspring resulting from gestational post-traumatic stress disorder
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DOI：
10.1016/j.bbr.2020.113026
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发表时间：
2021-02-05
影响因子：
2.7
通讯作者：
Vilela, Fabiana C.
中科院分区：
文献类型：
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作者：
Chagas, Luana A.;Batista, Tatiane H.;Vilela, Fabiana C.
围产期暴露在应激性环境事件中会增加精神病理的易感性，这些心理病理会导致神经内分泌变化，这些变化与可能在生命早期出现的情绪行为缺陷有关。创伤后应激障碍(PTSD)是一种频繁、慢性和致残的障碍，对受影响个人的情绪、社会和认知行为产生负面影响。因此，我们通过不可避免的足部电击诱导孕鼠创伤后应激障碍，然后研究青春期前类似于焦虑的子代的行为参数，以及母子代血浆皮质酮水平和子代海马糖皮质激素受体(GR)的表达。在水坝中，进行了母性行为、开阔场地和物体识别测试。对雄性和雌性仔鼠进行以下检查：超声发声定量、高架正迷宫试验、旷场探索活动评价、孔板试验、血浆皮质酮测定和Western blotting检测GR。我们的结果表明，妊娠期创伤后应激障碍影响母亲的行为，导致焦虑样症状，增加皮质酮水平，并增加子代海马区GR的表达。因此，我们的数据有助于理解由于在妊娠期暴露于创伤性事件而导致的早期(儿童期和青少年/青春期前)焦虑的发生。
Exposure to stressful environmental events during the perinatal period can increase vulnerability to psychopathologies that cause neuroendocrine changes associated with deficits in emotional behavior that can appear early in life. Post-traumatic stress disorder (PTSD) is a frequent, chronic, and disabling disorder that negatively impacts the emotional, social, and cognitive behaviors of affected individuals. Thus, we induced PTSD in pregnant rats by applying inescapable footshocks and then investigated the behavioral parameters similar to anxiety in offspring at prepubertal age, in addition to the plasma levels of maternal and offspring corticosterone and expression of glucocorticoid receptors (GR) in the offspring's hippocampus. With the dams, maternal behavior, open field, and object recognition tests were performed. With the male and female offspring, we performed the following: quantification of ultrasonic vocalizations, elevated plus-maze test, evaluation of exploratory activity in the open field, and hole board test, as well as plasma corticosterone measurements and Western blotting for GR. Our results showed that gestational PTSD affected maternal behavior, led to anxiety-like symptoms, increased corticosterone levels, and increased GR expression in the offspring's hippocampus. Therefore, our data can contribute to the understanding of the onset of early (childhood and juvenile/pre-pubertal phases) anxiety owing to exposure to a traumatic event during the gestation period.