Exon shuffling by L1 retrotransposition
Exon shuffling by L1 retrotransposition
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DOI：
10.1126/science.283.5407.1530
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发表时间：
1999-03-05
期刊：
影响因子：
56.9
通讯作者：
Kazazian, HH
中科院分区：
文献类型：
--
作者：
Moran, JV;DeBerardinis, RJ;Kazazian, HH
长分布的核元件(LINE-1S或L1S)是人类基因组中含量最丰富的反转录转座子，它们是逆转录酶活性的主要来源。在培养的人类细胞中高频率地进行工程L1s的逆转座。结果表明，L1插入到转录基因中，并将其3‘侧翼的逆转录转座序列插入到新的基因组位置。因此，逆转录转座能力的L1提供了一种载体，将非L1序列，如外显子或启动子，动员到现有的基因中，并可能代表新基因进化的一般机制。
Long interspersed nuclear elements (LINE-1s or L1s) are the most abundant retrotransposons in the human genome, and they serve as major sources of reverse transcriptase activity. Engineered L1s retrotranspose at high frequency in cultured human cells. Here it is shown that L1s insert into transcribed genes and retrotranspose sequences derived from their 3' flanks to new genomic Locations. Thus, retrotransposition-competent L1s provide a vehicle to mobilize non-L1 sequences, such as exons or promoters, into existing genes and may represent a general mechanism for the evolution of new genes.