Genetic diversity in seven perennial Ryegrass (Lolium perenne L.) cultivars based on SSR markers
Genetic diversity in seven perennial Ryegrass (Lolium perenne L.) cultivars based on SSR markers
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DOI：
10.2135/cropsci2001.4151565x
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发表时间：
2001-09-01
期刊：
影响因子：
2.3
通讯作者：
Gaut, BS
中科院分区：
文献类型：
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作者：
Kubik, C;Sawkins, M;Gaut, BS
品种鉴定的一个基本前提是确定品种是否存在遗传分化。研究了7种多年生黑麦草（Lolium perenne L.）品种（Loretta、Linn、曼哈顿II、Affinity、Jet、Penn-fine和Palmer III），目的是确定是否可以基于遗传数据区分品种。在每个品种中，我们用22个SSR标记对30个个体进行了基因分型，其中18个标记以前没有报道过。我们的研究结果表明，每一个品种都含有较高但相似的遗传多样性水平。品种内杂合度范围为0.589 - 0.643。这些品种可以通过一些统计标准来区分，包括：（i）小但重要的比例（2）品种F间的遗传变异显著，变异值在0.065~0.197之间;以及（iv）通过与判别分析相关的基因型分配测试来正确识别个体。基因型分配测试的效果特别好，它正确地将所有210个个体分配到它们的原产品种。抽样分析表明，基因型鉴定需要至少15个SSR标记的数据才能达到99%以上的准确率，这表明今后多年生黑麦草品种遗传多样性研究应至少使用15个SSR标记。总体而言，本文报道的SSR标记对品种间的区分是非常有效的。
An essential prerequisite to cultivar identification is to determine whether cultivars are differentiated genetically. We investigated genetic diversity among and within seven perennial ryegrass (Lolium perenne L.) cultivars (Loretta, Linn, Manhattan II, Affinity, Jet, Penn-fine, and Palmer III) using simple sequence repeat (SSR) markers, with the goal of determining whether cultivars could be differentiated on the basis of genetic data. In each cultivar we genotyped 30 individuals with 22 SSR markers, 18 of which had not been reported previously. Our results indicated that each of the seven cultivars contained high but similar levels of genetic diversity. Within cultivar heterozygosity ranged from 0.589 to 0.643. The cultivars could be distinguished by a number of statistical criteria, including: (i) a small but significant proportion (14.6%) of among-cultivar genetic variation, based on analysis of molecular variance (AMOVA); (ii) significant between cultivar F, values that ranged from 0.065 to 0.197; (iii) separation of individuals in principal component analysis (PCA); and (iv) correct identification of individuals by the genotype assignment test, which is related to discriminant analysis. The genotype assignment test worked particularly well; it correctly assigned all 210 individuals to their cultivar of origin. Sampling analysis indicated that the genotype assignment requires data from at least 15 SSRs to be >99% accurate, suggesting future studies of genetic diversity in perennial ryegrass cultivars should use at least 15 SSR markers. Overall, the SSRs reported in this paper were highly effective for differentiating among cultivars.