FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction.
FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction.
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DOI：
10.1016/j.bmcl.2009.09.122
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发表时间：
2009-12
影响因子：
2.7
通讯作者：
H. Nonaka;S. Fujishima;Sho-hei Uchinomiya;A. Ojida;I. Hamachi
中科院分区：
文献类型：
--
作者：
H. Nonaka;S. Fujishima;Sho-hei Uchinomiya;A. Ojida;I. Hamachi
本研究建立了FLAG标签选择性蛋白质标记方法。合成的Ni-复合物探针和FLAG标签之间的选择性结合与酰基转移反应的偶联使得能够对FLAG肽和FLAG-标签融合蛋白进行位点选择性共价修饰。
A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.