A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat
A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat
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DOI：
10.1016/j.jpba.2008.09.032
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发表时间：
2008-12-15
影响因子：
3.4
通讯作者：
Chen, Wansheng
中科院分区：
文献类型：
--
作者：
Cai, Fei;Sun, Lianna;Chen, Wansheng
建立了一种快速、灵敏、高选择性的液相色谱-串联质谱（LC-MS/MS）法测定知母皂苷B-II（TB-II）的含量。用该方法以铼为内标物，研究了TB-II在大鼠体内的药代动力学和生物利用度。在用乙腈对血浆样品进行简单的蛋白质沉淀后，在ODS柱（150 mm x 2.1 mm i.d.，5 μ m），以乙腈-水（35：65，v/v）（含0.05%甲酸）为移动的流动相，采用电喷雾电离质谱法在负多反应监测（MRM）模式下检测，色谱运行时间为3.0 min。在此范围内，相对标准偏差（R.S.D.）是
A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of Timosaponin B-II (TB-II), a pharmacologically active constituent isolated from Anemarrhena asphodeloides. This method was used to examine the pharmacokinetics and bioavailability of TB-II in rats using ginsenoside Re as an internal standard. After simple protein precipitation of the plasma samples with acetonitrile, the analytes were separated on an ODS column (150 mm x 2.1 mm i.d., 5 mu m) with the mobile phase of acetonitrile-water (35:65, v/v) containing 0.05% formic acid and detected by electrospray ionization mass spectrometry in the negative multiple reaction monitoring (MRM) mode with a chromatographic run time of 3.0 min. The calibration curves were linear over the range of 5-15,000 ng/ml and the lower limit of quantification (LLOQ) was 5 ng/ml in rat plasma. In this range, relative standard deviations (R.S.D.) were