A novel N-linked flagellar glycan from Methanococcus maripaludis
A novel N-linked flagellar glycan from Methanococcus maripaludis
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DOI：
10.1016/j.carres.2009.01.006
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发表时间：
2009-03-31
影响因子：
3.1
通讯作者：
Vinogradov, Evgeny
中科院分区：
文献类型：
--
作者：
Kelly, John;Logan, Susan M.;Vinogradov, Evgeny
古甲烷球菌Mm900菌株产生的鞭毛与N-连接的四糖糖基化。对鞭毛状胰蛋白酶多肽的质谱分析表明，含有质量为1036.4 Da的多糖，糖氧离子的裂解表明该多糖为四糖。在广泛的链霉蛋白酶消化鞭毛细丝后，通过大小排除和阴离子交换层析纯化了多糖。核磁共振波谱表明，该多糖具有如下结构：Sug-4-beta-ManNAc3NAmA6Thr-4-beta-Glc-NAc3NAcA-3-beta-GalNAc-Asn，其中Sug是一个新的单糖单元，(5S)-2-acetamido-2，4-dideoxy-5-O-methyl-alpha-L-erythro-hexos-5-ulo-1，5-pyranose.这种低聚糖与以前在甲烷球菌中发现的低聚糖有很大的相似性。(C)2009爱思唯尔有限公司。保留所有权利。
The archaea Methanococcus maripaludis strain Mm900 produces flagella that are glycosylated with an N-linked tetrasaccharide. Mass spectrometric analysis of flagellar tryptic peptides identified a number of tryptic glycopeptides carrying a glycan of mass 1036.4 Da, and fragmentation of the glycan oxonium, ion indicated that the glycan was a tetrasaccharide. The glycan was purified, following extensive pronase digestion of flagellar filaments, by size-exclusion and anion-exchange chromatography. NMR spectroscopy revealed that the glycan had the following structure: Sug-4-beta-ManNAc3NAmA6Thr-4-beta-Glc-NAc3NAcA-3-beta-GalNAc-Asn where Sug is a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-alpha-L-erythro-hexos-5-ulo-1,5-pyranose. This oligosaccharide has significant similarity to the oligosaccharide that was found previously in Methanococcus voltae. (C) 2009 Elsevier Ltd. All rights reserved.