Microarray analysis reveals that high mobility group A1 is involved in colorectal cancer metastasis.
Microarray analysis reveals that high mobility group A1 is involved in colorectal cancer metastasis.
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DOI：
10.3892/or.2013.2602
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发表时间：
2013-09
期刊：
影响因子：
4.2
通讯作者：
Y. Takahashi;G. Sawada;Tetsuya Sato;J. Kurashige;K. Mima;Tae Matsumura;R. Uchi;H. Ueo;M. Ishiba
中科院分区：
文献类型：
--
作者：
Y. Takahashi;G. Sawada;Tetsuya Sato;J. Kurashige;K. Mima;Tae Matsumura;R. Uchi;H. Ueo;M. Ishiba
肿瘤大小表明了大多数结直肠癌（CRC）病例中细胞增殖的程度，尽管有一些晚期小肿瘤伴有转移。淋巴结转移是影响结直肠癌预后的重要因素。导致CRC细胞淋巴结转移的潜在因素尚未完全了解。我们研究了可能诱导CRC转移的机制，重点是较小的尺寸（2 cm）无转移。使用来自另外77个病例和另一个172个病例的批量病例集的基因表达阵列数据验证结果。利用微阵列数据进行的基因本体和通路分析显示，抗凋亡活性在CRC转移中具有关键作用。高迁移率族A1（HMGA1）被确定为预后不良和转移形成的生物标志物。HMGA1表达水平在淋巴结阳性病例中高于淋巴结阴性病例，即使在粘膜下浸润病例的亚组分析中也是如此。本研究强烈支持HMGA1表达作为结直肠癌淋巴结转移的预测指标的临床意义，即使在粘膜下浸润的情况下，可以通过局部切除治愈。
Tumor size indicates the extent of cell proliferation in most cases of colorectal cancer (CRC), although there are some advanced small tumors with metastases. Lymph node metastasis is a significant factor that greatly impacts disease prognosis in CRC cases. The underlying factors that cause lymph node metastasis in CRC cells are not fully understood. We investigated the mechanism that might induce CRC metastasis by focusing on smaller sized (2 cm without metastases. Results were validated using gene expression array data from an additional 77 cases and another bulk case set of 172 cases. Gene ontology and pathway analysis using microarray data revealed that anti-apoptotic activity had a crucial role in CRC metastasis. High mobility group A1 (HMGA1) was identified as a biomarker for poor prognosis and metastasis formation. HMGA1 expression levels were higher in lymph node-positive cases than in lymph node-negative cases, even in subgroup analysis of submucosal invasive cases. The present study strongly supports the clinical significance of HMGA1 expression as a predictive indicator of lymph node metastasis in CRC cases, even in submucosal invasive cases which could be cured by local resection.