Sex-specific expression of alternative transcripts in Drosophila.
Sex-specific expression of alternative transcripts in Drosophila.
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DOI：
10.1186/gb-2006-7-8-r79
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发表时间：
2006
期刊：
影响因子：
12.3
通讯作者：
Nuzhdin SV
中科院分区：
文献类型：
--
作者：
McIntyre LM;Bono LM;Genissel A;Westerman R;Junk D;Telonis-Scott M;Harshman L;Wayne ML;Kopp A;Nuzhdin SV
果蝇性别特异性表达的替代转录的全基因组微阵列分析显示，53%的基因的转录丰度的性别二态性。许多基因由于选择性剪接或利用选择性转录起始/终止位点而产生多个转录物。这种“转录组扩增”被认为通过允许单个基因座产生几种功能不同的蛋白质来增加表型复杂性。然而，替代转录物表达中的性别、遗传和发育变异从未得到系统的研究。在这里，我们描述了一个全基因组分析性别特异性表达的替代转录果蝇。我们比较了男性和女性的8个果蝇系（OregonR和2B，和6 RIL）使用新设计的60聚体寡核苷酸微阵列，使我们能够区分很大比例的替代转录本的转录谱。新的微阵列包含7，207个寡核苷酸，满足严格的结合和特异性标准，靶向2，768个多转录基因的共同和独特区域，以及12，912个靶向具有单一已知转录物的基因的寡核苷酸。我们估计，高达22%的基因，产生多个转录本显示性别特异性偏见的替代转录本的代表。性二态性的总体转录丰度是明显的53%的基因。与常染色体相比，X染色体含有显著更高比例的具有女性偏向转录的基因。然而，X染色体上的基因并不比常染色体上的基因更容易在替代转录本表达中具有性别偏见。广泛的性别特异性表达的替代转录果蝇表明，在分子水平上存在一个新的水平的两性异形。
A genome-wide microarray analysis of sex-specific expression of alternative transcripts in Drosophila shows sexual dimorphism in transcript abundance for 53% of the genes. Many genes produce multiple transcripts due to alternative splicing or utilization of alternative transcription initiation/termination sites. This 'transcriptome expansion' is thought to increase phenotypic complexity by allowing a single locus to produce several functionally distinct proteins. However, sex, genetic and developmental variation in the representation of alternative transcripts has never been examined systematically. Here, we describe a genome-wide analysis of sex-specific expression of alternative transcripts in Drosophila melanogaster. We compared transcript profiles in males and females from eight Drosophila lines (OregonR and 2b, and 6 RIL) using a newly designed 60-mer oligonucleotide microarray that allows us to distinguish a large proportion of alternative transcripts. The new microarray incorporates 7,207 oligonucleotides, satisfying stringent binding and specificity criteria that target both the common and the unique regions of 2,768 multi-transcript genes, as well as 12,912 oligonucleotides that target genes with a single known transcript. We estimate that up to 22% of genes that produce multiple transcripts show a sex-specific bias in the representation of alternative transcripts. Sexual dimorphism in overall transcript abundance was evident for 53% of genes. The X chromosome contains a significantly higher proportion of genes with female-biased transcription than the autosomes. However, genes on the X chromosome are no more likely to have a sexual bias in alternative transcript representation than autosomal genes. Widespread sex-specific expression of alternative transcripts in Drosophila suggests that a new level of sexual dimorphism at the molecular level exists.