Calcium stimulation of a novel lipoxygenase.
Calcium stimulation of a novel lipoxygenase.
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新型脂氧合酶的钙刺激。
DOI：
10.1016/0006-291x(80)90710-x
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发表时间：
1980
影响因子：
3.1
通讯作者：
Steinhoff,MM
中科院分区：
文献类型：
--
作者：
Jakschik,BA;Sun,FF;Lee,L;Steinhoff,MM
大鼠嗜碱性白血病（RBL-1）细胞匀浆中有一种新的脂氧合酶，它能被钙以浓度依赖的方式激活，并被肾上腺素抑制。由[14 C]-花生四烯酸形成的主要化合物经气相色谱-质谱鉴定为5-羟基二十碳四烯酸和5，12-二羟基二十碳四烯酸。少量存在的其他化合物是12-羟基-和5，6-二羟基二十碳四烯酸。嗜碱性粒细胞中该途径的钙刺激将其与钙依赖性的释放反应紧密联系起来。
Homogenates of rat basophilic leukemia (RBL-1) cells have a novel lipoxygenase which was stimulated by calcium in a concentration dependent fashion and inhibited by epinephrine. The major compounds formed from [14C]-arachidonic acid were identified by gas chromatography — mass spectrometry to be 5-hydroxyeicosatetraenoic acid and 5,12-dihydroxyeicosatetraenoic acid. Other compounds present in small amounts were 12-hydroxy- and 5,6-dihydroxyeicosatetraenoic acid. The stimulation by calcium of this pathway in basophils links it closely to the release reaction which is calcium dependent.