Development of a Highly Sensitive Fluorescence Probe for Hydrogen Peroxide
Development of a Highly Sensitive Fluorescence Probe for Hydrogen Peroxide
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DOI：
10.1021/ja203521e
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发表时间：
2011-07-13
影响因子：
15
通讯作者：
Nagano, Tetsuo
中科院分区：
文献类型：
--
作者：
Abo, Masahiro;Urano, Yasuteru;Nagano, Tetsuo
过氧化氢被认为通过蛋白质的可逆氧化在细胞信号转导中发挥作用。在这里，我们报告了一种新型过氧化氢荧光探针的设计和合成，利用基于苯偶酰化学的光诱导电子转移策略来控制荧光。这种高度灵敏和选择性的荧光探针 NBzF 的实用价值通过其在活 RAW 264.7 巨噬细胞中过氧化氢生成成像的应用得到证实。 NBzF 还用于 A431 人表皮样癌细胞中作为信号分子产生的过氧化氢的活细胞成像。
Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.