Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell
Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell
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DOI：
10.1007/s00253-011-3181-y
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发表时间：
2011-05-01
影响因子：
5
通讯作者：
Premier, Giuliano C.
中科院分区：
文献类型：
--
作者：
Kim, Jung Rae;Beecroft, Nelli J.;Premier, Giuliano C.
在由两个串联模块组成的纵向管状微生物燃料电池反应器中，研究了细菌群落在产电生物膜中的时空发展。利用聚合酶链反应-变性梯度凝胶电泳（DGGE）和DNA测序技术对微生物群落组成的比例变化进行了评估，以将其与生物电化学系统的性能和稳定性联系起来。通过聚类分析和Jaccard’s系数对重复反应器的再现性进行了评价，结果表明重复反应器的物种组成相似度为80 ~ 90%。通过补料分批启动和随后的稳定连续操作，生物膜的发展导致种群从γ -变形菌门和拟杆菌门向厚壁菌门为主的群落转变，其他不同物种的相对比例要低得多。通过范围加权丰富度（Rr）和Pareto-Lorenz均匀分布曲线分析DGGE模式，探讨细菌群落的进化。第一个模块从连续模式运行开始时以与溶禾本科拟杆菌（Bacteroides graminisolvens）、溶鸟拉乌尔菌（Raoultella ornithinolytica）和克雷伯菌（Klebsiella sp. BM21）密切相关的物种为主转变为在第97天以丙酸古杆菌（Paludibacter propionigenes -）、乳球菌（Lactococcus sp.-）、Pantoea agglomerans-和克雷伯菌（Klebsiella oxytoca）相关的物种为主，并具有稳定的发电能力（6.0 W/m(3)）。考虑种群动态的操作策略将为微生物燃料电池的实际应用中评估系统性能提供有用的参数。
The spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. The proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) and DNA sequencing in order to relate them to the performance and stability of the bioelectrochemical system. The reproducibility of duplicated reactors, evaluated by cluster analysis and Jaccard's coefficient, shows 80-90% similarity in species composition. Biofilm development through fed-batch start-up and subsequent stable continuous operation results in a population shift from gamma-Proteobacteria- and Bacteroidetes- to Firmicutes-dominated communities, with other diverse species present at much lower relative proportions. DGGE patterns were analysed by range-weighted richness (Rr) and Pareto-Lorenz evenness distribution curves to investigate the evolution of the bacterial community. The first modules shifted from dominance by species closely related to Bacteroides graminisolvens, Raoultella ornithinolytica and Klebsiella sp. BM21 at the start of continuous-mode operation to a community dominated by Paludibacter propionicigenes-, Lactococcus sp.-, Pantoea agglomerans- and Klebsiella oxytoca-related species with stable power generation (6.0 W/m(3)) at day 97. Operational strategies that consider the dynamics of the population will provide useful parameters for evaluating system performance in the practical application of microbial fuel cells.