Tissue engineering of corneal endothelium.
Tissue engineering of corneal endothelium.
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DOI：
10.3390/jfb3040726
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发表时间：
2012-10-17
影响因子：
4.8
通讯作者：
Yamagami S
中科院分区：
文献类型：
--
作者：
Mimura T;Yokoo S;Yamagami S
人角膜内皮细胞（HCECs）在受伤后不复制。因此，角膜内皮缺乏可导致不可逆的角膜水肿。Descemet剥离自动内皮角膜移植术（DSAEK）允许选择性替代病变角膜内皮。然而，DSAEK需要一个供体角膜，世界范围内角膜的短缺限制了它的应用。本文综述了利用培养的HCECs进行角膜内皮组织工程的最新研究进展。我们还介绍了我们最近在利用培养的HCECs进行DSAEK移植物组织工程方面的工作。我们通过在胶原片上播种培养的dii标记的HCECs来重建DSAEK移植物。DSAEK组术后将HCEC片移植到后基质上。对照组在观察期间出现严重间质水肿，而DSAEK组在观察期间未出现严重间质水肿。术后1个月荧光显微镜显示DSAEK组角膜后表面有大量dii标记细胞。冷冻切片显示在Descemet的膜上有一层dii标记的细胞。这些结果表明，体外培养的成人HCECs经DSAEK手术移植后，可维持移植后角膜的透明度，并提示采用DSAEK联合HCECs治疗内皮功能障碍的可行性。
Human corneal endothelial cells (HCECs) do not replicate after wounding. Therefore, corneal endothelial deficiency can result in irreversible corneal edema. Descemet stripping automated endothelial keratoplasty (DSAEK) allows selective replacement of the diseased corneal endothelium. However, DSAEK requires a donor cornea and the worldwide shortage of corneas limits its application. This review presents current knowledge on the tissue engineering of corneal endothelium using cultured HCECs. We also provide our recent work on tissue engineering for DSAEK grafts using cultured HCECs. We reconstructed DSAEK grafts by seeding cultured DiI-labelled HCECs on collagen sheets. Then HCEC sheets were transplanted onto the posterior stroma after descemetorhexis in the DSAEK group. Severe stromal edema was detected in the control group, but not in the DSAEK group throughout the observation period. Fluorescein microscopy one month after surgery showed numerous DiI-labelled cells on the posterior corneal surface in the DSAEK group. Frozen sections showed a monolayer of DiI-labelled cells on Descemet’s membrane. These findings indicate that cultured adult HCECs, transplanted with DSAEK surgery, maintain corneal transparency after transplantation and suggest the feasibility of performing DSAEK with HCECs to treat endothelial dysfunction.