The impact of postnatal antibiotics on the preterm intestinal microbiome
The impact of postnatal antibiotics on the preterm intestinal microbiome
复制标题
DOI：
10.1038/pr.2014.69
复制
发表时间：
2014-08-01
影响因子：
3.6
通讯作者：
Guillet, Ronnie
中科院分区：
文献类型：
--
作者：
Dardas, Majd;Gill, Steven R.;Guillet, Ronnie
背景：早产儿肠道微生物群的发育对婴儿健康有重要影响。我们的目的是确定出生后10天和30天内持续使用抗生素是否会影响肠道微生物。方法：受试者为胎龄24 0/7-31 6/7周，在10天前摄入50%母乳和总计100毫升/公斤的饲料，在10天和30天采集直肠(粪便)拭子，进行16S rRNA焦磷酸测序分析。在这两个时间点，我们检查了只接受2d抗生素治疗的婴儿和接受至少7d抗生素治疗的婴儿的直肠微生物群。结果：在我们研究的29名婴儿中，我们发现接受更多抗生素治疗的婴儿的多样性指数从10d样本开始下降。这种多样性和丰富度的差异在30d的样本中并不明显。在10d的样品中，菌丝和拟杆菌数量最多。虽然这两个门在30d的样本中仍占主导地位，但变形杆菌和放线菌有所增加。结论：尽管接受了抗生素治疗，新生儿胃肠道仍有细菌感染。抗生素的使用扰乱了细菌的获取过程。
BACKGROUND: Development of the intestinal microbiome in preterm infants has significant impact on infant health. Our objective was to determine if duration of antibiotics within the first 10 and 30 d after birth affects the intestinal microbiome.METHODS: Subjects were 24 0/7-31 6/7 wk of gestational age who received >= 50% breast milk and a total of 100 ml/kg of feeds by 10 d. Rectal (fecal) swabs were collected at 10 and 30 d and analyzed by 16S rRNA pyrosequencing. At both time points, we examined the rectal microbiome from infants who received only 2 d of antibiotics and those who received at least 7 d of antibiotics.RESULTS: In the 29 infants enrolled in our study, we found a decrease in diversity index from 10 d samples in those who received more antibiotics. Such difference in diversity and richness was not as pronounced in 30 d samples. Firmicutes and Bacteroidetes were most abundant in the 10 d samples. While these two phyla remained dominant in 30 d samples, there was an increase in Proteobacteria and Actinobacteria.CONCLUSION: Despite antibiotic therapy, neonates continued to acquire bacteria in the gastrointestinal tract. The process of bacterial acquisition is perturbed with the use of antibiotics.