A cytorhabdovirus phosphoprotein forms mobile inclusions trafficked on the actin/ER network for viral RNA synthesis
A cytorhabdovirus phosphoprotein forms mobile inclusions trafficked on the actin/ER network for viral RNA synthesis
复制标题
细胞弹状病毒磷蛋白形成在肌动蛋白/ER网络上运输的移动内含物，用于病毒RNA合成
DOI：
10.1093/jxb/erz195
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发表时间：
2019-08-01
影响因子：
6.9
通讯作者：
Wang, Xian-Bing
中科院分区：
文献类型：
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作者：
Fang, Xiao-Dong;Yan, Teng;Wang, Xian-Bing
摘要植物病毒作为专性寄生物，通常劫持宿主细胞骨架进行复制和移动。弹状病毒是一种有包膜的负链RNA病毒，感染脊椎动物、无脊椎动物和植物，但植物弹状病毒蛋白的细胞内运输机制在很大程度上尚不清楚。在这里，我们使用大麦黄纹花叶病毒（BYSMV），一种植物细胞弹状病毒，作为一个模型，研究肌动蛋白细胞骨架对病毒的细胞内运动和病毒RNA合成的微型复制子（MR）系统的影响。BYSMV P蛋白形成移动的包涵体，其沿肌动蛋白/内质网网络沿着运输，并将N和L蛋白募集到病毒质样结构中。缺失分析表明，BYSMV P的N端区域（aa 43-55）和剩余区域（aa 56-295）分别对包涵体的移动和形成起重要作用。肌球蛋白XI-K尾的过度表达完全消除了P体的运输活性，并伴随着病毒MR RNA合成的显著减少。这些结果表明，BYSMV P有助于形成和运输病毒质样结构沿着ER/肌动蛋白网络驱动的肌球蛋白XI-K。因此，弹状病毒P似乎是一种动态的枢纽蛋白，用于有效募集病毒蛋白，从而促进病毒RNA合成。
Abstract As obligate parasites, plant viruses usually hijack host cytoskeletons for replication and movement. Rhabdoviruses are enveloped, negative-stranded RNA viruses that infect vertebrates, invertebrates, and plants, but the mechanisms of intracellular trafficking of plant rhabdovirus proteins are largely unknown. Here, we used Barley yellow striate mosaic virus (BYSMV), a plant cytorhabdovirus, as a model to investigate the effects of the actin cytoskeleton on viral intracellular movement and viral RNA synthesis in a mini-replicon (MR) system. The BYSMV P protein forms mobile inclusion bodies that are trafficked along the actin/endoplasmic reticulum network, and recruit the N and L proteins into viroplasm-like structures. Deletion analysis showed that the N terminal region (aa 43–55) and the remaining region (aa 56–295) of BYSMV P are essential for the mobility and formation of inclusions, respectively. Overexpression of myosin XI-K tails completely abolishes the trafficking activity of P bodies, and is accompanied by a significant reduction of viral MR RNA synthesis. These results suggest that BYSMV P contributes to the formation and trafficking of viroplasm-like structures along the ER/actin network driven by myosin XI-K. Thus, rhabdovirus P appears to be a dynamic hub protein for efficient recruitment of viral proteins, thereby promoting viral RNA synthesis.