Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics
Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics
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DOI：
10.1016/j.bios.2004.02.020
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发表时间：
2004-10-15
影响因子：
12.6
通讯作者：
Heath, JR
中科院分区：
文献类型：
--
作者：
Pantoja, R;Nagarah, JM;Heath, JR
我们报告了一种基于硅晶片的装置，可用于记录宏观离子通道蛋白在不同细胞类型的活动。CHO-K1和RIN m5 F细胞实现了千兆欧姆密封，并且还证明了细胞附着和全细胞模式配置。在全细胞模式下，HIT-T15和RAW 264.7细胞记录到两种不同的内源性钾离子通道。聚二甲基硅氧烷（PDMS）微流体也与微加工的硅芯片耦合，以证明单个细胞可以选择性地定向到细胞，随后可以记录膜蛋白电流。这些基于硅芯片的设备具有显着的优势，传统的微量移液管的方法，并可以作为组合工具，用于调查膜生物物理学，药物筛选，和其他生物传感任务。(C)2004 Elsevier B. V.保留所有权利。
We report on a silicon wafer-based device that can be used for recording macroscopic ion channel protein activities across a diverse group of cell-types. Gigaohm seals were achieved for CHO-K1 and RIN m5F cells, and both cell-attached and whole-cell mode configurations were also demonstrated. Two distinct intrinsic potassium ion channels were recorded in whole-cell mode for HIT-T15 and RAW 264.7 cells. Polydimethylsiloxane (PDMS) microfluidics were also coupled with the micromachined silicon chips in order to demonstrate that a single cell could be selectively directed to a micropore, and membrane protein currents could subsequently be recorded. These silicon chip-based devices have significant advantages over traditional micropipette approaches, and may serve as combinatorial tools for investigating membrane biophysics, pharmaceutical screening, and other bio-sensing tasks. (C) 2004 Elsevier B.V. All rights reserved.