Development of high-performance liquid chromatographic for quality and authenticity control of Chinese propolis.
Development of high-performance liquid chromatographic for quality and authenticity control of Chinese propolis.
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DOI：
10.1111/1750-3841.12510
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发表时间：
2014-07
影响因子：
3.9
通讯作者：
Cui-ping Zhang;H. Shuai;Wen-ting Wei;Ping Shun;Xiao-ge Shen;Ya-jing Li;Fu-liang Hu
中科院分区：
文献类型：
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作者：
Cui-ping Zhang;H. Shuai;Wen-ting Wei;Ping Shun;Xiao-ge Shen;Ya-jing Li;Fu-liang Hu
建立了同时测定蜂胶中12种黄酮类成分和8种酚酸类物质的反相高效液相色谱分析方法。结果表明，在所分析的蜂胶和杨树树胶样品中均未检测到香草酸、芦丁、杨梅素和木犀草素。在杨树胶中未检测到咖啡酸、阿魏酸和对香豆酸，而在蜂胶中检测到咖啡酸、阿魏酸和对香豆酸，表明它们是区分蜂胶和杨树胶的实用指标。杨树胶中的黄酮类成分与蜂胶相似，主要由松脂素、松脂素、3-O-乙酰基松脂素、白杨素和高良姜素组成。因此，以咖啡酸、阿魏酸、对香豆酸为定性指标，可将杨树胶从蜂胶中剔除，并可将杨树胶与其他非法物质区分开来，以松黄素、松膜素、3-O-乙酰松黄素、白杨素、高良姜素为定性和定量指标，对杨树胶的质量进行分级。
A RP-high-performance liquid chromatography (HPLC) method was developed for quality control of Chinese propolis by simultaneous analysis of 12 flavonoids and 8 phenolic acids. The results showed that vanillic acid, rutin, myricetin, and luteolin were not detected in all of the analyzed propolis and poplar tree gum samples. The caffeic acid, ferulic acid and p-coumaric acid were not detected in poplar tree gum but were detected in propolis, which suggest that they are practical indexes of distinguishing propolis from poplar tree gum. The flavonoid profiles of poplar tree gum were found to be similar to those of propolis, which are dominated by pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin. Therefore, the proposed method could be applied to exclude poplar tree gum from propolis with cafferic acid, ferulic acid, and p-coumaric acid as qualitative markers, and distinguish poplar source resin from other illegal substances, and evaluate the quality grading of poplar-type propolis with pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin as qualitative and quantitative markers.