Different collagen types define two types of idiopathic epiretinal membranes
Different collagen types define two types of idiopathic epiretinal membranes
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DOI：
10.1111/j.1365-2559.2011.03820.x
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发表时间：
2011-05-01
期刊：
影响因子：
6.4
通讯作者：
Hillenkamp, Jost
中科院分区：
文献类型：
--
作者：
Kritzenberger, Michaela;Junglas, Benjamin;Hillenkamp, Jost
目的：探讨特发性玻璃纸黄斑反射视网膜前膜(IEM)与视网膜前黄斑纤维化(PMFM)之间细胞外基质含量的差异。方法与结果：对特发性视网膜前膜进行光镜、定量电子显微镜、免疫组织化学及Western blotting分析。在细胞外纤维的性质方面，CMRM和PMFM有很大的不同。CMRM组纤维较细，直径在6~15 nm之间。纤维之间可见长间隔的胶原蛋白聚集体。PMFM中纤维直径为18~26 nm或36~56 nm。免疫金电子显微镜下，CMRM中6-15 nm的纤维被标记为VI型胶原，而PMFM中的纤维未被染色。免疫组织化学和Western blotting结果显示，所有CMRM均表达VI型胶原，PMFM免疫反应较弱或不表达。相比之下，PMFM显示I型和II型胶原的免疫反应阳性，而CMRM中I型和II型胶原较弱或缺失。两种膜对III型和IV型胶原、层粘连蛋白和纤维连接蛋白的免疫反应强度相似。结论：CMRM中存在大量的VI型胶原，而相对缺乏I型和II型胶原是PMFM的主要结构差异。
Different collagen types define two types of idiopathic epiretinal membranesAims:To identify differences in extracellular matrix contents between idiopathic epiretinal membranes (IEM) of cellophane macular reflex (CMRM) or preretinal macular fibrosis (PMFM) type.Methods and results:Idiopathic epiretinal membranes were analysed by light and quantitative transmission electron microscopy, immunohistochemistry and Western blotting. Substantial differences between CMRM and PMFM were observed regarding the nature of extracellular fibrils. In CMRM the fibrils were thin, with diameters between 6 and 15 nm. Between the fibrils, aggregates of long-spacing collagen were observed. In PMFM the diameters of fibrils measured either 18-26 or 36-56 nm. Using immunogold electron microscopy, 6-15 nm fibrils in CMRM were labelled for collagen type VI, while the fibrils in PMFM remained unstained. Using Western blotting and immunohistochemistry, a strong signal for collagen type VI was observed in all CMRM, while immunoreactivity was weak or absent in PMFM. In contrast, PMFM showed immunoreactivity for collagen types I and II, which was weak or absent in CMRM. Both types of membranes showed immunoreactivity for collagen types III and IV, laminin and fibronectin with similar intensity.Conclusion:The presence of high amounts of collagen type VI in CMRM and the relative absence of collagen types I and II is the major structural difference to PMFM.