Automated Single-Particle Reconstruction of Heterogeneous Inorganic Nanoparticles
Automated Single-Particle Reconstruction of Heterogeneous Inorganic Nanoparticles
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DOI：
10.1017/s1431927620024642
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发表时间：
2020-12-01
影响因子：
2.8
通讯作者：
Allen, Christopher S.
中科院分区：
文献类型：
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作者：
Slater, Thomas J. A.;Wang, Yi-Chi;Allen, Christopher S.
单粒子重建可用于执行纳米尺寸物体的均匀群体的三维（3D）成像，特别是病毒和蛋白质。在这里，它表明，它也可以用于获得无机纳米粒子的异质群体的3D重建。扫描透射电子显微镜中的自动采集方案用于收集数千个纳米颗粒的图像。粒子图像随后根据其属性进行半自动聚类，并从选定的粒子图像聚类中执行单独的3D重建。结果是一个代表整个人群的3D数据集。该研究展示了一种方法，该方法允许以完全自动化的方式对无机纳米颗粒进行3D成像和分析，真正代表了大颗粒群。
Single-particle reconstruction can be used to perform three-dimensional (3D) imaging of homogeneous populations of nano-sized objects, in particular viruses and proteins. Here, it is demonstrated that it can also be used to obtain 3D reconstructions of heterogeneous populations of inorganic nanoparticles. An automated acquisition scheme in a scanning transmission electron microscope is used to collect images of thousands of nanoparticles. Particle images are subsequently semi-automatically clustered in terms of their properties and separate 3D reconstructions are performed from selected particle image clusters. The result is a 3D dataset that is representative of the full population. The study demonstrates a methodology that allows 3D imaging and analysis of inorganic nanoparticles in a fully automated manner that is truly representative of large particle populations.