Applications of Fourier transform infrared microspectroscopy in studies of benign prostate and prostate cancer. A pilot study
Applications of Fourier transform infrared microspectroscopy in studies of benign prostate and prostate cancer. A pilot study
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DOI：
10.1002/path.1421
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发表时间：
2003-09-01
影响因子：
7.3
通讯作者：
Brown, M
中科院分区：
文献类型：
--
作者：
Gazi, E;Dwyer, J;Brown, M
傅里叶变换红外 (FTIR) 显微光谱已应用于研究来自不同转移部位的前列腺癌细胞系以及良性前列腺组织和格里森分级的恶性前列腺组织。将石蜡包埋的组织样本安装到 BaF2 板上，然后使用 Citroclear 和丙酮去除蜡后，通过 FTIR 进行分析。将细胞系作为保持在两个 BaF2 板之间的细胞悬浮液的等分试样进行分析。研究发现，1030 cm(-1) 和 1080 cm(-1) 处的峰面积比分别对应于糖原和磷酸盐振动，提示了一种区分良性细胞和恶性细胞的潜在方法。该比率与 FTIR 光谱成像的结合使用为估计样本定义区域内的恶性组织面积提供了基础。使用线性判别算法对 FTIR 光谱进行初始化学计量处理，展示了一种用于良性和恶性组织分类以及格里森分级 CaP 光谱分离的有前途的方法。本研究利用主成分分析，首次实现了来自不同转移部位的前列腺癌细胞系的 FTIR 光谱的分离。版权所有 (C) 2003 John Wiley Sons, Ltd.
Fourier transform infrared (FTIR) microspectroscopy has been applied to a study of prostate cancer cell lines derived from different metastatic sites and to tissue from benign prostate and Gleason-graded malignant prostate tissue. Paraffin-embedded tissue samples were analysed by FTIR, after mounting onto a BaF2 plate and subsequent removal of wax using Citroclear followed by acetone. Cell lines were analysed as aliquots of cell suspension held between two BaF2 plates. It was found that the ratio of peak areas at 1030 and 1080 cm(-1), corresponding to the glycogen and phosphate vibrations respectively, suggests a potential method for the differentiation of benign from malignant cells. The use of this ratio in association with FTIR spectral imaging provides a basis for estimating areas of malignant tissue within defined regions of a specimen. Initial chemometric treatment of FTIR spectra, using the linear discriminant algorithm, demonstrates a promising method for the classification of benign and malignant tissue and the separation of Gleason-graded CaP spectra. Using the principle component analysis, this study has achieved for the first time the separation of FTIR spectra of prostate cancer cell lines derived from different metastatic sites. Copyright (C) 2003 John Wiley Sons, Ltd.