On line characterization of 58 phenolic compounds in Citrus fruit juices from Spanish cultivars by high-performance liquid chromatography with photodiode-array detection coupled to electrospray ionization triple quadrupole mass spectrometry
On line characterization of 58 phenolic compounds in Citrus fruit juices from Spanish cultivars by high-performance liquid chromatography with photodiode-array detection coupled to electrospray ionization triple quadrupole mass spectrometry
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DOI：
10.1016/j.talanta.2012.05.042
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发表时间：
2012-09-15
期刊：
影响因子：
6.1
通讯作者：
Vicente, Francisca
中科院分区：
文献类型：
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作者：
Abad-Garcia, Beatriz;Garmon-Lobato, Sergio;Vicente, Francisca
采用高效液相色谱-二极管阵列检测器-电喷雾离子化-三重四极杆质谱联用技术，对西班牙甜橙子、柑橘、柠檬和葡萄柚等柑橘类果汁中的多酚类物质进行了分析。共鉴定出5类58种酚类化合物。黄烷酮：柚皮素O-二己糖苷;黄酮类：芹菜素-7-O-芸香糖苷-4'-O-葡萄糖苷、毛地黄黄酮-7-O-新橙皮糖苷-4'-O-葡萄糖苷、毛地黄黄酮-6-C-葡萄糖苷、黄菊醇和薯蓣皂苷的6，8-二-C-酰基己糖苷、芹菜素的6C-和-8C-葡萄糖苷-O-戊糖苷、芹菜素-6-C-己糖苷-O-己糖苷和芹菜素-8-C-己糖苷-O-酰基鼠李糖苷;黄酮醇：槲皮素、山奈酚、异鼠李素和柽柳素的7-O-芸香糖苷，山奈酚-3-O-芸香糖苷，异鼠李素-3-O-芸香糖苷-7-O-葡萄糖苷，柽柳素-3-O-芸香糖苷-7-O-葡萄糖苷，异鼠李素-3-O-己糖苷-7-O-鼠李糖基己糖苷，槲皮素和异鼠李素的3-O-鼠李糖苷-7-O-鼠李糖基己糖苷和山奈酚-3-O-鼠李糖基己糖苷-7-O-鼠李糖苷;羟基肉桂酸：阿魏酸和芥子酸的O-己糖苷;和香豆素：东莨菪亭的O-己糖苷和O-鼠李糖基己糖苷，据我们所知，以前在柑橘汁中没有报道。结构已分配的基础上获得的保留时间，紫外-可见光谱，扫描模式MS光谱，并在MS2光谱使用不同的碰撞能量的碎片模式的互补信息。为不同酚类化合物的结构鉴定提供了一种结构诊断方案。(C)2012爱思唯尔有限公司版权所有。
Polyphenol profile of Citrus juices of sweet orange, tangerine, lemon and grapefruit from Spanish cultivars was obtained by High-Performance Liquid Chromatography with Diode Array Detection coupled to Electrospray ionization and Triple Quadrupole Mass Spectrometry. Fifty eight phenolic compounds of five different classes were identified in these Citrus juices. Flavanone: O-dihexoside of naringenin; flavones: apigenin-7-O-rutinoside-4'-O-glucoside, luteolin-7-O-neohesperidoside-4'-O-glucoside, luteolin-6-C-glucoside, 6,8-di-C-acylhexosides of chrysoeriol and diosmetin, 6C- and -8C-glucoside-O-pentoside of apigenin, apigenin-6-C-hexoside-O-hexoside and apigenin-8-C-hexoside-O-acylrhamnoside; flavonols: 7-O-rutinosides of quercetin, kaempferol, isorhamnetin and tamarixetin, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside-7-O-glucoside, tamarixetin-3-O-rutinoside-7-O-glucoside, isorhamnetin-3-O-hexoside-7-O-rhamnosylhexoside, 3-O-rhamnoside-7-O-rhamnosylhexoside of quercetin and isorhamnetin and kaempferol-3-O-rhamnosylhexoside-7-O-rhamnoside; hydroxycinnamic acids: O-hexoside of ferulic and sinapic acid; and, coumarins: O-hexoside and O-rhamnosylhexoside of scopoletin, had not previously been reported in Citrus juices to our knowledge. Structures have been assigned on the basis of the complementary information obtained from retention time, UV-visible spectra, scan mode MS spectra, and fragmentation patterns in MS2 spectra obtained using different collision energies. A structure diagnosis scheme is provided for the identification of different phenolic compounds. (C) 2012 Elsevier B.V. All rights reserved.