The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.
The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.
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DOI：
10.1016/s0021-9258(18)70158-0
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发表时间：
1959-04
期刊：
影响因子：
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通讯作者：
A. Weissbach;J. Hurwitz
中科院分区：
文献类型：
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作者：
A. Weissbach;J. Hurwitz
Waravdekar和Saslaw(1)报道了脱氧核糖的敏感颜色试验，该试验依赖于脱氧糖的高碘酸氧化。在这种氧化过程中形成的丙二醛与硫代巴比妥酸偶联，得到吸收最大值为532 rnp的显色原。在研究大肠杆菌B株提取物中5-磷酸核糖的代谢时，注意到在高碘酸-硫代巴比妥酸试验中反应产物的形成和积累。在这种情况下形成的颜色在545至550 rnp而不是532 mp处具有最大吸收。产生这种颜色的化合物已被确定为2-酮-3-脱氧庚酸(I)，其中碳4、5和6上基团的构型仍未确定。
Waravdekar and Saslaw (1) have reported a sensitive color test for deoxyribose which depends on the periodate oxidation of the deoxy sugar. The malondialdehyde formed in this oxidation is coupled with thiobarbituric acid to give a chromogen with an absorption maximum at 532 rnp. While investigating the metabolism of ribose 5-phosphate in extracts of Escherichia coli strain B, the formation and accumulation of a product which reacted in the periodate-thiobarbituric acid test was noticed. The color formed in this case had an absorption maximum at 545 to 550 rnp rather than 532 mp. The compound responsible for the color has been identified as 2-keto-3-deoxyheptonic acid (I) in which the configuration of the groups on carbons 4, 5, and 6 remains undetermined.