Resistant dextrin improves high-fat-high-fructose diet induced insulin resistance
Resistant dextrin improves high-fat-high-fructose diet induced insulin resistance
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DOI：
10.1186/s12986-020-00450-2
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发表时间：
2020-05-15
影响因子：
4.5
通讯作者：
Zhang, Hongmei
中科院分区：
文献类型：
--
作者：
Hu, Fan;Niu, Yixin;Zhang, Hongmei
背景：胰岛素抵抗是与肥胖和2型糖尿病相关的重要缺陷。许多研究报道膳食纤维具有有益的代谢作用。抗糊精是一种可溶性纤维。本研究旨在探讨耐药糊精对高脂高果糖饮食诱导的肥胖小鼠的影响，并探讨其机制。方法选取17只4周龄雄性C57BL/ 6j小鼠，分别饲喂正常日粮（ND）和HFHFD，连续22周，灌胃5 g/kg抗性糊精，连续10周。进行葡萄糖耐量试验（GTT）和胰岛素耐量试验（ITT），测定血清空腹胰岛素（FINS）和血清生化指标，酶法测定肝组织甘油三酯（TG）和总胆固醇（TC）含量。HE染色检测肝脏病理变化。采用Real time PCR和Western blot分别检测胰岛素信号通路和脂肪酸β氧化通路相关基因和蛋白的表达。通过16s rRNA测序分析肠道菌群。结果抗药糊精显著降低小鼠血清FINS，改善血脂，降低肝脏TG和TC含量。胰岛素信号通路和脂肪酸氧化通路被促进。耐药糊精组肠道菌群中代谢有益菌如普雷沃氏菌和阿克曼氏菌的丰度增加。结论耐药糊精能显著改善肝脏胰岛素抵抗，改善血脂水平，减少肝脏脂质沉积。肠道菌群的调节可能是耐药糊精产生有益作用的原因。
Background Insulin resistance is an important defect associated with obesity and type 2 diabetes mellitus. Many studies have been reported that dietary fiber exerts beneficial metabolic effects. Resistant dextrin is a soluble fiber. The aim of this study was to investigate the effects of resistant dextrin on high-fat-high-fructose diet induced obese mice and to explore the underlying mechanisms. Methods Seventeen 4-week-old male C57BL/6 J mice were fed a normal diet (ND) or HFHFD for 22 weeks, and were gavaged with resistant dextrin (5 g/kg) for 10 weeks. Glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed, serum fasting insulin (FINS) and serum biochemical parameters were determined, the contents of triglyceride (TG) and total cholesterol (TC) in liver tissues were determined by enzymatic method. The pathological changes in liver were detected by HE staining. Real time PCR and Western blot were used to detect the expression of insulin signaling pathway and the fatty acid beta oxidation pathway related genes and proteins respectively. The gut microbiota were analyzed via 16 s rRNA sequencing. Results Resistant dextrin significantly decreased serum FINS, improved serum lipid profiles, reduced the contents of liver TG and TC. The insulin signaling pathway and the fatty acid beta oxidation pathway were promoted. The abundance of metabolically beneficial bacteria such as Prevotella and Akkermansia in the intestinal flora of the resistant dextrin group were increased. Conclusions Resistant dextrin can significantly ameliorate liver insulin resistance, improve serum lipid levels, as well as reduce hepatic lipid deposition. The modulation of gut microbiota might be responsible for the beneficial effects of resistant dextrin.