Differences in Antibody Responses Between Trivalent Inactivated Influenza Vaccine and Live Attenuated Influenza Vaccine Correlate With the Kinetics and Magnitude of Interferon Signaling in Children
Differences in Antibody Responses Between Trivalent Inactivated Influenza Vaccine and Live Attenuated Influenza Vaccine Correlate With the Kinetics and Magnitude of Interferon Signaling in Children
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DOI：
10.1093/infdis/jiu079
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发表时间：
2014-07-15
影响因子：
6.4
通讯作者：
Ramilo, Octavio
中科院分区：
文献类型：
--
作者：
Cao, Raquel G.;Suarez, Nicolas M.;Ramilo, Octavio
背景流感减毒活疫苗（LAIV）和三价流感灭活疫苗（TIV）可有效预防儿童流感病毒感染，但其保护机制尚不清楚。我们分析了6个月至14岁儿童接种这两种疫苗后B细胞反应和转录谱的差异。LAIV在接种后第30天引起幼稚、记忆和过渡B细胞的显著增加，而TIV在第7天引起浆母细胞数量的增加。TIV组中针对3种疫苗株（H1 N1、H3 N2和B）的抗体滴度显著更高，并且与抗体分泌细胞的数量相关。两种疫苗诱导干扰素（IFN）信号转导基因的过度表达，但具有不同的动力学。在所有年龄组中，TIV在接种后第1天诱导IFN基因表达，LAIV在接种后第7天诱导IFN基因表达，但仅在儿童中
Background. Live attenuated influenza vaccine (LAIV) and trivalent inactivated influenza vaccine (TIV) are effective for prevention of influenza virus infection in children, but the mechanisms associated with protection are not well defined.Methods. We analyzed the differences in B-cell responses and transcriptional profiles in children aged 6 months to 14 years immunized with these 2 vaccines.Results. LAIV elicited a significant increase in naive, memory, and transitional B cells on day 30 after vaccination, whereas TIV elicited an increased number of plasmablasts on day 7. Antibody titers against the 3 vaccine strains (H1N1, H3N2, and B) were significantly higher in the TIV group and correlated with number of antibody-secreting cells. Both vaccines induced overexpression of interferon (IFN)-signaling genes but with different kinetics. TIV induced expression of IFN genes on day 1 after vaccination in all age groups, and LAIV induced expression of IFN genes on day 7 after vaccination but only in children