Analysis of electro-mechanical relationship in human iPS cell-derived cardiomyocytes sheets under proarrhythmic condition assessed by simultaneous field potential and motion vector recordings.
Analysis of electro-mechanical relationship in human iPS cell-derived cardiomyocytes sheets under proarrhythmic condition assessed by simultaneous field potential and motion vector recordings.
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通过同步场电位和运动矢量记录评估促心律失常条件下人类 iPS 细胞来源的心肌细胞片的机电关系分析。
DOI：
10.1016/j.jphs.2019.07.006
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Izumi-Nakaseko H
中科院分区：
文献类型：
--
作者：
Sugiyama A;Hagiwara-Nagasawa M;Kambayashi R;Goto A;Chiba K;Naito AT;Kanda Y;Matsumoto A;Izumi-Nakaseko H
我们研究了人iPS细胞来源的心肌细胞片层在缺氧条件下的电-机械关系，这是由洋地黄中毒沿着电序列刺激诱导的（n = 4）。我们采用高速视频显微镜的运动矢量分析和64微电极阵列系统的细胞外场电位记录。运动矢量分析揭示了静息期的局部收缩事件，在静息期，场电位分析显示任何细胞片均无偏转。因此，运动矢量分析可以提供额外的信息，现场电位记录在检测钙触发的心律失常，这可能成为一个新的战略，评估供试品的药物敏感性。
We investigated the electro-mechanical relationship of human iPS cell-derived cardiomyocyte sheets under arrhythmic condition, which was induced by digitalis intoxication along with the electrical train stimulation (n = 4). We adopted motion vector analysis by high-speed video microscopy and extracellular field potential recording by 64-microelectrode array system. The motion vector analysis uncovered local contractile events at resting phase, at which the field potential analysis showed no deflection in any cell sheet. Thus, motion vector analysis may provide supplemental information over field potential recording in detecting Ca2+-triggered arrhythmias, which may become a new strategy for assessing arrhythmic liability of test articles.