TWO HUMAN TNF RECEPTORS HAVE SIMILAR EXTRACELLULAR BUT DISTINCT INTRACELLULAR DOMAIN SEQUENCES
TWO HUMAN TNF RECEPTORS HAVE SIMILAR EXTRACELLULAR BUT DISTINCT INTRACELLULAR DOMAIN SEQUENCES
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DOI：
10.1016/1043-4666(90)90022-l
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发表时间：
1990-01-01
期刊：
影响因子：
3.8
通讯作者：
LESSLAUER W
中科院分区：
文献类型：
--
作者：
DEMBIC Z;LOETSCHER H;LESSLAUER W
肿瘤坏死因子（TNF）是一种在炎症和免疫反应中具有广泛生物学活性的细胞因子。这些活性由55 kDa和75 kDa表观分子量的特异性细胞表面受体介导。一个75-kDa的TNF受体cDNA分离使用部分氨基酸序列信息和聚合酶链反应（PCR）。当在COS-1细胞中表达时，cDNA转移特异性TNF-结合特性与天然受体相当。预测的细胞外区域包含四个结构域，其特征性半胱氨酸残基与55-kDa TNF受体、神经生长因子（NGF）受体以及CDw 40和OX 40抗原的半胱氨酸残基高度相似。TNF受体胞外结构域的共有序列也与富含半胱氨酸的序列基序LIM具有相似性。与细胞外区域形成鲜明对比的是，两种TNF受体的细胞内结构域是完全不相关的，表明不同的信号传导和功能模式。
Tumor necrosis factor (TNF) is a cytokine with a wide range of biological activities in inflammatory and immunologic responses. These activities are mediated by specific cell surface receptors of 55 kDa and 75 kDa apparent molecular masses. A 75-kDa TNF receptor cDNA was isolated using partial amino acid sequence information and the polymerase chain reaction (PCR). When expressed in COS-1 cells, the cDNA transfer specific TNF-binding properties comparable to those of the native receptor. The predicted extracellular region contains four domains with characteristic cysteine residues higly similar to those of the 55-kDa TNF receptor, the nerve growth factor (NGF) receptor, and the CDw40 and OX40 antigens. The consensus sequence of the TNF receptor extracellular domains also has similarity to the cysteine-rich sequence motif LIM. In marked contrast to the extracellular regions, the intracellular domains of the two TNF receptors are entirely unrelated, suggesting different modes of signaling and function.