TUMOR SELECTIVE ENHANCEMENT OF RADIOACTIVITY UPTAKE IN MICE TREATED WITH ALPHA-DIFLUOROMETHYLORNITHINE PRIOR TO ADMINISTRATION OF C-14-LABELED PUTRESCINE
TUMOR SELECTIVE ENHANCEMENT OF RADIOACTIVITY UPTAKE IN MICE TREATED WITH ALPHA-DIFLUOROMETHYLORNITHINE PRIOR TO ADMINISTRATION OF C-14-LABELED PUTRESCINE
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DOI：
10.1016/0024-3205(83)90193-5
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发表时间：
1983-01-01
期刊：
影响因子：
6.1
通讯作者：
DIGENIS, GA
中科院分区：
文献类型：
--
作者：
CHANEY, JE;KOBAYASHI, K;DIGENIS, GA
一组携带EMT 6肿瘤的雄性BALB/c小鼠，其已经用α-二氟甲基鸟氨酸（DFMO，一种特异性的、不可逆的鸟氨酸脱羧酶（催化腐胺生物合成的酶）抑制剂），8 mg/小鼠，腹膜内，在14 C-腐胺给药前20和5小时，对一组对照动物施用14 C-腐胺（0.5 μ Ci，0.1 mCi/mmol，i. v.）处死前60分钟。获得了肿瘤和13个主要正常器官[胰腺、肝脏、脾脏、肾脏、胃、十二指肠、小肠、肌肉、心脏、肺、脑、睾丸、血液]的放射性摄取数据。在对照动物中，肿瘤显示出最高的放射性摄取。对于DFMO预处理的小鼠，大多数正常组织中的放射性分布与对照动物的放射性分布差异不大。肿瘤的摄取增强了约1倍。4. DFMO治疗的结果是肿瘤与组织的比例较高（肺：3.8;脑：38）。DFMO与14 C-腐胺在肿瘤成像的有用性进行了讨论。
A group of EMT6 tumor bearing male BALB/c mice which had been treated with .alpha.-difluoromethylornithine (DFMO, a specific, irreversible inhibitor of ornithine decarboxylase, the enzyme which catalyzes the biosynthesis of putrescine), 8 mg/mouse, i.p., 20 and 5 h before the 14C-putrescine dose, and a group of control animals were administered 14C-putrescine (0.5 .mu.Ci, 0.1 mCi/mmol, i.v.) 60 min prior to sacrifice. Radioactivity uptake data was obtained for the tumor and 13 major normal organs [pancreas, liver, spleen, kidney, stomach, duodenum, small intestine, muscle, heart, lung, brain, testes, blood]. In the control animals the tumor exhibited one of the highest uptakes of radioactivity. For DFMO-pretreated mice the radioactivity distribution among most of the normal tissues was not very different from that obtained for the control animals. The uptake into the tumor was enhanced by a factor of .apprx. 4. High tumor-to-tissue ratios (3.8, lung to 38, brain) were attained as a result of DFMO treatment. The usefulness of DFMO with 14C-putrescine in imaging tumors is discussed.