Exploring the root-associated microbiome of the resurrection plant Myrothamnus flabellifolia
Exploring the root-associated microbiome of the resurrection plant Myrothamnus flabellifolia
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DOI：
10.1007/s11104-023-06019-1
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发表时间：
2023-05-10
期刊：
影响因子：
4.9
通讯作者：
Farrant，Jill M. M.
中科院分区：
文献类型：
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作者：
Tebele，Shandry M. M.;Marks，Rose A. A.;Farrant，Jill M. M.
目的和背景复苏植物Myrothamnus flabellifoliatolerate完全干燥，是一个伟大的模式，研究植物如何科普极端干旱。根际微生物在作物的抗逆性中起着重要的作用，是提高作物耐旱性的一个有吸引力的目标。然而，在最极端的水资源限制下，这些动态是如何发挥作用的，仍然没有得到充分的探索。本研究的目的是确定细菌和真菌群落的耐受极端干旱胁迫的大部分土壤，根际，和内圈的M。方法采用高通量扩增子测序技术对M. flabellifolia的微生物群落进行分析。结果细菌门在所有区室中最丰富的是酸杆菌门、放线菌门、绿弯菌门、浮菌门和假单胞菌门，而真菌门最丰富的是子囊菌门和担子菌门。虽然散装土壤托管多种有益的根相关微生物，根际区室表现出最高的功能多样性的细菌和真菌。相比之下，内圈的微生物丰度和多样性较低。这些发现与M. flabellifolia将土壤微生物从本体招募到根际，最后招募到内圈。这是可能的，这些微生物可以促进耐旱相关的植物tissues.ConclusionWe发现，车厢作为微生物多样性的主要驱动程序，但土壤理化因素也影响微生物组成。这些结果表明，根相关的微生物ofM。扇叶高度结构化，并可有助于植物功能。
Aims and backgroundThe resurrection plantMyrothamnus flabellifoliatolerates complete desiccation and is a great model for studying how plants cope with extreme drought. Root-associated microbes play a major role in stress tolerance and are an attractive target for enhancing drought tolerance in staple crops. However, how these dynamics play out under the most extreme water limitation remains underexplored. This study aimed to identify bacterial and fungal communities that tolerate extreme drought stress in the bulk soil, rhizosphere, and endosphere ofM. flabellifolia.MethodsHigh-throughput amplicon sequencing was used to characterise the microbial communities associated withM. flabellifolia.ResultsThe bacterial phyla that were most abundant across all compartments wereAcidobacteriota, Actinobacteriota, Chloroflexota, Planctomycetota,andPseudomonadota, while the most abundant fungal phyla wereAscomycotaandBasidiomycota. Although the bulk soil hosted multiple beneficial root-associated microbes, the rhizosphere compartment showed the highest functional diversity of bacteria and fungi. In contrast, the endosphere exhibited a low abundance and diversity of microbes. These findings share consistent with the theory thatM. flabellifoliarecruits soil microbes from the bulk to the rhizosphere and finally to the endosphere. It is possible that these microbes could promote drought tolerance in associated plant tissues.ConclusionWe find that compartments act as the major driver of microbial diversity, but the soil physicochemical factors also influence microbial composition. These results suggest that the root-associated microbiome ofM. flabellifoliais highly structured and may aid in plant function.