White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP)
White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP)
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DOI：
10.3354/dao074171
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发表时间：
2007-03-13
影响因子：
1.4
通讯作者：
Huang, Wei-Pang
中科院分区：
文献类型：
--
作者：
Chen, Li-Li;Lu, Li-Chen;Huang, Wei-Pang
白色斑点综合征病毒（White Spot Syndrome Virus，WSSV）是养殖对虾的一种严重病害。利用纯化的WSSV病毒颗粒，经SDS-PAGE和蛋白质组学鉴定，VP 53 A为WSSV 067开放阅读框编码的结构蛋白。金标二抗免疫电镜显示VP 53 A分布在病毒包膜上。为了进一步研究WSSV 067与宿主蛋白质之间的联系，我们以WSSV 067 C为诱饵，对斑节对虾cDNA文库进行了酵母双杂交筛选。与WSSV 067 C特异性相互作用的分子之一是斑节对虾几丁质结合蛋白（PmCBP）。体外结合试验表明，c-myc-WSSV 067 C能够共沉淀HA-PmCBP-C。此外，PmCBP几乎在所有器官中表达，但似乎在WSSV感染后期上调。
White spot syndrome virus (WSSV) is the causative agent of a severe disease of cultivated shrimp. Using purified WSSV virions, VP53A encoded by open reading frame wssv067 was identified as a structural protein by SDS-PAGE and proteomics. Immunoelectron microscopy with a gold-labeled secondary antibody revealed that VP53A was distributed on the viral envelope. ln order to further explore the link between WSSV067 and host proteins, we performed a yeast 2-hybrid screening of a Penaeus monodon cDNA library, using WSSV067C as bait. One of the molecules that specifically interacted with WSSV067C was the P. monodon chitin-binding protein (PmCBP). An in vitro binding assay showed that c-myc-WSSV067C was capable of co-precipitating HA-PmCBP-C. Furthermore, PmCBP was expressed in almost all organs but appeared to be up-regulated at the late stage of WSSV infection.